Single-Pair Courtship and Competition Assays in Drosophila
Single-Pair Courtship and Competition Assays in Drosophila
复制标题
果蝇的单对求爱和竞争测定
DOI：
10.1101/pdb.prot108105
复制
发表时间：
2023
影响因子：
--
通讯作者：
Von Philipsborn A
中科院分区：
文献类型：
--
作者：
Von Philipsborn A
黑腹果蝇的求偶涉及一系列先天的复杂行为，这些行为允许雄性和雌性果蝇交换感官信息并评估潜在配偶的质量。虽然求爱行为是高度稳健和刻板的，但它可以受到内部状态和经验的调节。该方案描述了设计和进行实验的方法，这些实验在单对测定中测量求偶性能，在单对测定中，雄性与雌性配对，或者在竞争测定中，雄性与雌性和雄性竞争者一起呈现。它还包括测量女性在求爱期间的性接受能力的方法。
Courtship in Drosophila melanogaster involves a series of innate, complex behaviors that allow male and female flies to exchange sensory information and assess the quality of a potential mate. Although highly robust and stereotypical, courtship behaviors can be modulated by internal state and experience. This protocol describes methods for designing and carrying out experiments that measure courtship performance in single-pair assays, in which a male is paired with a female, or in competitive assays, in which a male is presented with a female and with a male competitor. It also includes approaches for measuring female sexual receptivity during courtship.