Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach.
Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach.
复制标题
通过完整的腺病毒介导的方法产生重组腺相关病毒载体。
DOI：
10.1006/mthe.2001.0306
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Li,CY
中科院分区：
文献类型：
--
作者：
Zhang,X;Li,CY
大规模生产rAAV的有效方法将显著促进这种有前途的基因递送载体在人类基因治疗应用中的应用。在这项研究中，我们报告了一种新的方法，rAAV的生产是完全基于腺病毒载体，而不需要质粒转染和特殊的包装细胞系。通过腺病毒载体将rAAV生产所需的所有组分（包括重组腺病毒基因和治疗基因）递送至广泛使用的293包装细胞系。通过使用该方法获得高滴度rAAV载体（200-600感染单位/生产细胞）。由于腺病毒载体可以产生高滴度，并且它们可以有效地感染悬浮液中的细胞，因此这种方法可以适用于rAAV载体的规模化生产。
An efficient approach to the large-scale production of rAAV will significantly facilitate the application of this promising gene delivery vector in human gene therapy applications. In this study, we report a novel approach to rAAV production that is based exclusively on the adenovirus vector, without the need for plasmid transfections and special packaging cell lines. All components required for rAAV production, including therepandcapgenes, and the therapeutic gene(s) are delivered to the widely used 293 packaging cell line by adenovirus vectors. High-titer rAAV vectors (200–600 infectious units/producer cell) were obtained by use of this approach. As adenovirus vectors can be produced to high titers and they can infect cells in suspension efficiently, this approach may be amenable to scaled-up production of rAAV vectors.