cDNA microarray analysis of rat alveolar epithelial cells following exposure to organic extract of diesel exhaust particles
cDNA microarray analysis of rat alveolar epithelial cells following exposure to organic extract of diesel exhaust particles
复制标题
DOI：
10.1016/j.taap.2004.05.006
复制
发表时间：
2004-12-01
影响因子：
3.8
通讯作者：
Kobayashi, T
中科院分区：
文献类型：
--
作者：
Koike, E;Hirano, S;Kobayashi, T
柴油机排气颗粒物（DEP）可诱发哮喘和慢性支气管炎等肺部疾病。要了解包括DEP在内的空气污染物对肺部疾病的影响机制，需要进行全面的评价。使用cDNA微阵列，我们研究了在SV 40 T2细胞，大鼠肺泡II型上皮细胞系，暴露于有机提取物的DEP后的基因表达的变化。我们确定了候选敏感基因，上调或下调响应DEP。cDNA微阵列分析显示，暴露于DEP提取物（30 μ g/ml）6小时后，与药物代谢、抗氧化、细胞周期/增殖/凋亡、凝血/纤溶和表达相关的51个基因的表达增加（>2倍）序列标签（EST），并减少（
Diesel exhaust particles (DEP) induce pulmonary diseases including asthma and chronic bronchitis. Comprehensive evaluation is required to know the mechanisms underlying the effects of air pollutants including DEP on lung diseases. Using a cDNA microarray, we examined changes in gene expression in SV40T2 cells, a rat alveolar type II epithelial cell line, following exposure to an organic extract of DEP. We identified candidate sensitive genes that were up- or down-regulated in response to DEP. The cDNA microarray analysis revealed that a 6-h exposure to the DEP extract (30 mug/ml) increased (>2-fold) the expression of 51 genes associated with drug metabolism, antioxidation, cell cycle/proliferation/apoptosis, coagulation/fibrinolysis, and expressed sequence tags (ESTs), and decreased (