Structural and functional characterization of the mouse Hlx homeobox gene
Structural and functional characterization of the mouse Hlx homeobox gene
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DOI：
10.1007/s003350010179
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发表时间：
2000-10-01
期刊：
影响因子：
2.5
通讯作者：
Potter, SS
中科院分区：
文献类型：
--
作者：
Bates, MD;Schatzman, LC;Potter, SS
Hlx是一种间质表达的同源异型盒转录因子基因，对小鼠肠和肝的正常发育至关重要。在这里，我们报告进一步表征的小鼠Hlx基因，包括一个额外的3.7 kb的5'端序列以及三个内含子的序列。将小鼠Hlx基因5'端编码区的序列与人基因的序列进行比较，揭示了多个高度保守的区域。无论是小鼠还是人类基因都不包含TATA盒，核糖核酸酶保护研究定义了小鼠基因的异质性转录起始位点。在小鼠和人Hlx基因之间，在高度保守区域之内和之外，许多共有转录因子结合位点是保守的。含有4.2或1.4kb的小鼠5'序列的报告构建体在表达Hlx的细胞系中显示出活性表达。小鼠Hlx基因的进一步表征将提供深入了解小鼠消化系统的发育调控。
Hlx is a mesenchymally expressed homeobox transcription factor gene that is essential for normal intestinal and hepatic development in the mouse. Here we report further characterization of the mouse Hlx gene, including an additional 3.7 kb of 5' sequence as well as the sequence of the three introns. Comparison of the sequence of the mouse Hlx gene 5' to the coding region with that of the human gene revealed multiple regions of high conservation. Neither the mouse nor the human gene contained a TATA box, and ribonuclease protection studies defined heterogeneous transcription start sites for the mouse gene. A number of consensus transcription factor binding sites were conserved between the mouse and human Hlx genes both within and outside of the highly conserved regions. Reporter constructs containing 4.2 or 1.4 kb of mouse 5' sequence showed active expression in cell lines that express Hlx. Further characterization of the mouse Hlx gene will provide insight into the developmental regulation of the mouse digestive system.