Doppler fluctuation spectroscopy of intracellular dynamics in living tissue
Doppler fluctuation spectroscopy of intracellular dynamics in living tissue
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DOI：
10.1364/josaa.36.000665
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发表时间：
2019-04-01
影响因子：
1.9
通讯作者：
Nolte, David D.
中科院分区：
文献类型：
--
作者：
Li, Zhe;Sun, Hao;Nolte, David D.
活组织的胞内动力学主要是由远离热平衡的生物能过程驱动的主动运输。细胞内成分通常执行持续行走。在长平均自由程的极限下，持续的行走是弹道的，在光散射波动光谱中表现出“多普勒边缘”。在较短的输运长度下，波动用寿命展宽的多普勒谱来描述。动态光散射从传输在弹道，扩散，或交叉制度是解析推导，包括自相关函数的推导通过驱动阻尼谐振振荡器模拟光散射从持续行走。通过蒙特卡罗仿真验证了该理论。实验证据的多普勒边缘在三维（3D）活组织使用生物动力成像基于低相干干涉测量和数字全息。(C) 2019美国光学学会
Intracellular dynamics in living tissue are dominated by active transport driven by bioenergetic processes far from thermal equilibrium. Intracellular constituents typically execute persistent walks. In the limit of long mean free paths, the persistent walks are ballistic, exhibiting a "Doppler edge" in light scattering fluctuation spectra. At shorter transport lengths, the fluctuations are described by lifetime-broadened Doppler spectra. Dynamic light scattering from transport in the ballistic, diffusive, or the crossover regimes is derived analytically, including the derivation of autocorrelation functions through a driven damped harmonic oscillator analog for light scattering from persistent walks. The theory is validated through Monte Carlo simulations. Experimental evidence for the Doppler edge in three-dimensional (3D) living tissue is obtained using biodynamic imaging based on low-coherence interferometry and digital holography. (C) 2019 Optical Society of America