Effect of vitamin B-6 deficiency on an antibody production in mice
Effect of vitamin B-6 deficiency on an antibody production in mice
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DOI：
10.1271/bbb.61.1331
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发表时间：
1997-08-01
影响因子：
1.6
通讯作者：
Tsuge, H
中科院分区：
文献类型：
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作者：
Doke, S;Inagaki, N;Tsuge, H
为了研究维生素B-6（B-6）缺乏对BALB/c小鼠抗体产生的影响，仅通过酶联免疫吸附测定的方法测量抗二硝基苯基化卵清蛋白（DNP-OVA）的特异性免疫球蛋白（Ig）E抗体的产生，将在缺乏维生素B的饮食下喂养几周的小鼠用氢氧化铝凝胶吸附的DNP-OVA进行腹膜内免疫，测定抗DNP-IgE抗体的含量与喂食含有 B-6 的饮食的对照小鼠相比，B-6 缺陷小鼠的血清中的 IgE 显着增加，此外，已知可诱导 B-6 缺陷小鼠脾细胞过敏反应中产生 IgE 的白细胞介素 4 (Interleukin-4) 大约比对照小鼠高出四倍。脾脏重量、血清总蛋白、IgG 和抗 DNP-IgE 含量恢复到与对照几乎相同的水平。这些结果表明小鼠 B-6 缺乏可能与刺激针对 DNP-OVA 的特异性 IgE 抗体产生有关。
To investigate the effects of vitamin B-6 (B-6) deficiency on an antibody production in BALB/c mice, the production of specific immunoglobulin (Ig) E antibody against dinitrophenylated ovalbumin (DNP-OVA) mere measured by the methods of enzyme linked immunosorbent assay, The mice fed on on a B, deficient diet for it weeks were immunized intraperitoneally with DNP-OVA absorbed to aluminum hydroxide gel, The contents of anti DNP-IgE antibodies in sera of B-6 deficient mice significantly increased compared to that of control mice fed on a diet containing B-6, In addition, Interleukin-4, which was known to induce IgE production in allergic reactions from splenocytes of B-6 deficient mice, was approximately four-fold higher than that in control mice,According to the recovery test to the B-6 deficient mice, that is feeding the control diet for 21 days, all values in terms of the body, thymus, and spleen weight, total serum protein, IgG, and anti DNP-IgE content, regained almost the same levels as those of control, These results suggest that B-6 deficiency in mice would have relation to the stimulation of specific IgE antibody production against DNP-OVA.