Biosurfactant Production from Lactobacilli: an Insight on the Interpretation of Prevailing Assessment Methods
Biosurfactant Production from Lactobacilli: an Insight on the Interpretation of Prevailing Assessment Methods
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DOI：
10.1007/s12010-021-03686-7
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发表时间：
2021-09-24
影响因子：
3
通讯作者：
Kopsahelis, Nikolaos
中科院分区：
文献类型：
--
作者：
Kachrimanidou, Vasiliki;Papadaki, Aikaterini;Kopsahelis, Nikolaos
生物表面活性剂由两亲性分子组成，作为环境友好的、可生物降解的替代品在食品、制药和化妆品应用中取代石油衍生的对应物而受到越来越多的关注。然而，它们的高生产成本阻碍了工业化生产。在这项研究中，50 GRAS乳酸杆菌菌株进行了筛选，他们的能力，生产生物表面活性剂，实现不同的基板。奶酪乳清渗透物（CWP）也被评估为低成本和固有的乳酸杆菌底物，旨在减轻其污染影响，扩大增值战略，减轻来自商业补充剂的成本，提高整体可持续性。采用表面张力、乳化活性（E-24）和驱油能力来确定最有前途的候选物。结果表明，表面张力作为最强大的方法，并强调基板上的生物表面活性剂合成的影响。同样，这项研究表明，在菌株选择过程中，包括最终发酵底物（CWP），以避免误解的结果，并提高后续的生物过程集成的基本作用。
Biosurfactants constitute amphiphilic molecules, receiving increased attention as environmentally benign, biodegradable alternatives to substitute for the petroleum derived counterparts in food, pharmaceutical and cosmetics applications. However, their high production cost hinders industrial production. In this study, fifty GRAS lactobacilli strains were screened for their ability to produce biosurfactants, implementing different substrates. Cheese whey permeate (CWP) was also assessed as a low-cost and inherent lactobacilli substrate, aiming to mitigate its polluting impact, expand valorization strategies, alleviate costs deriving from commercial supplements and enhance overall sustainability. Surface tension, emulsification activity (E-24) and oil displacement were deployed to identify the most promising candidates. Results reveal surface tension as the most robust method and underline the effect of substrate on biosurfactant synthesis. Likewise, this study indicates the fundamental role of including the final fermentation substrate (CWP) during strain selection to avoid misinterpretation of results and enhance subsequent bioprocess integration.