Downregulation of RASAL2 promotes the proliferation, epithelial-mesenchymal transition and metastasis of colorectal cancer cells
Downregulation of RASAL2 promotes the proliferation, epithelial-mesenchymal transition and metastasis of colorectal cancer cells
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DOI：
10.3892/ol.2017.5581
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发表时间：
2017-03-01
期刊：
影响因子：
2.9
通讯作者：
Xiao, Zhongfu
中科院分区：
文献类型：
--
作者：
Jia, Zeming;Liu, Weidong;Xiao, Zhongfu
RAS蛋白激活物样2（RASAL 2）是一种RAS-GTP酶激活蛋白，最近已被鉴定为多种类型人类癌症的肿瘤抑制因子;然而，RASAL 2在结直肠癌（CRC）中的功能仍不清楚。在本研究中，使用RASAL 2功能丧失细胞模型研究了RASAL 2在CRC细胞中的功能。将RASAL 2短发夹RNA转染到人CRC细胞系LoVo、SW 620和HCT 116以及野生型结肠细胞系NCM 460中。随后的下调RASAL 2进行了评估，使用蛋白质印迹和逆转录定量聚合酶链反应分析。RASAL 2在人结直肠癌组织和细胞系中的表达显著降低（P
RAS protein activator like 2 (RASAL2) is a RAS-GTPase-activating protein and has recently been identified to be a tumor suppressor in various types of human cancer; however, the function of RASAL2 in colorectal carcinoma (CRC) remains unclear. In the present study, the function of RASAL2 in CRC cells was investigated using a RASAL2 loss-of-function cell model. RASAL2 short hairpin RNA was transfected into the human CRC cell lines LoVo, SW620 and HCT116, and the wild-type colon cell line NCM460. The subsequent downregulation of RASAL2 was evaluated using western blot and reverse transcription-quantitative polymerase chain reaction analyses. It was observed that RASAL2 expression was significantly decreased in human CRC tissues and cell lines (P