Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis
Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis
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DOI：
10.1038/nature04794
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发表时间：
2006-05-25
期刊：
影响因子：
64.8
通讯作者：
Amon, Angelika
中科院分区：
文献类型：
--
作者：
Brar, Gloria A.;Kiburz, Brendan M.;Amon, Angelika
在减数分裂期间，内聚蛋白——将姐妹染色单体连接在一起的蛋白质复合物——以一种循序渐进的方式从染色体上消失(1)。染色体臂上黏结蛋白的丢失是同源染色体在减数分裂i期间分离所必需的。着丝粒周围黏结蛋白的保留直到减数分裂II是姐妹染色单体准确分离所必需的。本研究表明，内聚蛋白亚基Rec8的磷酸化有助于逐步去除内聚蛋白。我们的数据进一步暗示了减数分裂染色体分离的另外两个关键调节因子，即黏附蛋白保护剂Sgo1和减数分裂重组，导致了黏附蛋白的逐步丢失，从而建立了减数分裂染色体分离模式。了解这些过程之间的相互作用，将有助于深入了解减数分裂染色体错误分离背后的事件，而减数分裂染色体错误分离是人类流产和智力迟钝的主要原因。
During meiosis, cohesins-protein complexes that hold sister chromatids together-are lost from chromosomes in a step-wise manner(1). Loss of cohesins from chromosome arms is necessary for homologous chromosomes to segregate during meiosis I. Retention of cohesins around centromeres until meiosis II is required for the accurate segregation of sister chromatids. Here we show that phosphorylation of the cohesin subunit Rec8 contributes to step-wise cohesin removal. Our data further implicate two other key regulators of meiotic chromosome segregation, the cohesin protector Sgo1 and meiotic recombination in bringing about the step-wise loss of cohesins and thus the establishment of the meiotic chromosome segregation pattern. Understanding the interplay between these processes should provide insight into the events underlying meiotic chromosome mis-segregation, the leading cause of miscarriages and mental retardation in humans.