Comparative transcriptomics reveals suppressed expression of genes related to auxin and the cell cycle contributes to the resistance of cucumber against Meloidogyne incognita.
Comparative transcriptomics reveals suppressed expression of genes related to auxin and the cell cycle contributes to the resistance of cucumber against Meloidogyne incognita.
复制标题
比较转录组学揭示生长素相关基因的表达受到抑制，细胞周期有助于黄瓜对南方根结线虫的抗性
DOI：
10.1186/s12864-018-4979-0
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发表时间：
2018-08-03
期刊：
影响因子：
4.4
通讯作者：
Chen JF
中科院分区：
文献类型：
--
作者：
Wang X;Cheng C;Zhang K;Tian Z;Xu J;Yang S;Lou Q;Li J;Chen JF
研究背景南方根结线虫（Meloidogyne incogniti）是一种毁灭性的线虫，在世界范围内对黄瓜生产造成重大损失。虽然许多研究都强调植物对线虫感染的敏感性反应，但M.黄瓜的抗隐孢子虫性仍然难以捉摸。用M.黄瓜对隐叶病的抗性研究为阐明黄瓜对隐叶病的抗性机制提供了机会。结果分析了IL 10 -1（抗性株系）和CC 3（感病株系）在M.不知名的感染生理学观察显示巨细胞和M. Incognitain IL 10 -1，这是与CC 3相比的主要差异。此外，基因本体论（GO）分析显示，编码细胞壁蛋白的基因在IL 10 -1中上调，并且高表达的脂质转运蛋白基因（Csa 6 G410090）可能是这种上调的主要调节因子。同时，基因表达谱分析显示，更多的生长素相关基因被抑制在IL 10 -1比在CC 3，这对应于较低的吲哚乙酸（IAA）的水平比在CC 3的根。此外，作为IL 10 -1中异常巨细胞的明确指示的较差的细胞核发育与细胞周期的抑制有关。在与细胞周期相关的基因中，F-box结构域Skp 2样基因在IL 10 -1中表达下调，而在CC 3中表达上调的基因较多。结论生长素相关基因和细胞周期相关基因的表达下调以及细胞壁蛋白的高表达在巨细胞发育异常中起重要作用，从而阻碍了巨细胞的发育。Incognita，从而引起对M的抗性。Incognitain IL 10 -1。从这项研究的知识将提供一个有用的基础，制定有效的战略在M。抗隐名病育种
BackgroundMeloidogyne incognitais a devastating nematode that causes significant losses in cucumber production worldwide. Although numerous studies have emphasized on the susceptible response of plants after nematode infection, the exact regulation mechanism ofM. incognita-resistance in cucumber remains elusive. Verification of an introgression line, ‘IL10–1’, withM. incognita-resistance provides the opportunity to unravel the resistance mechanism of cucumber againstM. incognita.ResultsIn the present study, analyses of physiological responses and transcriptional events between IL10–1 (resistant line) and CC3 (susceptible line) were conducted afterM. incognitainfection. Physiological observations showed abnormal development of giant cells andM. incognitain IL10–1, which were the primary differences compared with CC3. Furthermore, Gene ontology (GO) analysis revealed that genes encoding cell wall proteins were up-regulated in IL10–1 and that the highly expressed lipid transfer protein gene (Csa6G410090) might be the principal regulator of this up-regulation. Simultaneously, analyses of gene expression profiles revealed more auxin-related genes were suppressed in IL10–1 than in those of CC3, which corresponded with the lower level of indole acetic acid (IAA) in the roots of IL10–1 than in those of CC3. Additionally, poor nucleus development as a clear indication of abnormal giant cells in IL10–1 was related to inhibition of the cell cycle. Of those genes related to the cell cycle, the F-box domain Skp2-like genes were down-regulated in IL10–1, whereas more of these genes were up-regulated in CC3.ConclusionsAll of these findings indicate that suppressed expression of genes related to auxin and the cell cycle and highly expressed cell wall proteins play important roles in the abnormal development of giant cells, which hinders the development ofM. incognita,thereby causing resistance toM. incognitain IL10–1. Knowledge from this research will provide a useful foundation for developing effective strategies inM. incognita-resistance breeding.