Cell attachment activity of cementum: bone sialoprotein II identified in cementum.
Cell attachment activity of cementum: bone sialoprotein II identified in cementum.
复制标题
牙骨质的细胞附着活性：在牙骨质中鉴定出骨唾液蛋白II。
DOI：
10.1111/j.1600-0765.1991.tb01620.x
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发表时间：
1991
影响因子：
3.5
通讯作者：
Argraves,WS
中科院分区：
文献类型：
--
作者：
Somerman,MJ;Sauk,JJ;Foster,RA;Norris,K;Dickerson,K;Argraves,WS
相当多的研究工作都是针对准备根表面的方式，将促进细胞附着导致牙周再生；然而，没有任何方法被证明是临床可预测的。鉴定与牙骨质根表面基质相关的附着蛋白可能对开发有效的临床治疗有价值。本研究采用胍/EDTA连续朝向萃取法分别从牛和人的牙齿中提取牙骨质蛋白。胍/EDTA提取物，而不是胍提取物，被发现促进成纤维细胞的附着。这种附着活性被含有精氨酸-甘氨酸-天冬氨酸（RGD）的合成肽抑制。对胍/EDTA提取物进行分离，发现有几个部分具有附着活性。免疫印迹分析表明，其中两个部分含有骨相关的RGD附着蛋白，骨唾液蛋白- II （BSP - II）。此外，在其他不能归因于BSP - II或纤维连接蛋白的组分中也发现了附着活性。这些研究表明，骨水泥的附着活性的一个组成部分可能是由于BSP‐II，并且骨水泥含有额外的，尚未确定的附着蛋白。
Considerable research effort has been directed at preparing root surfaces in a fashion that would promote cell attachment leading to periodontal regeneration; however, no methods have proven to be clinically predictable. Identification of attachment protein(s) associated with the root surface matrix of cementum may prove valuable for developing effective clinical treatments. In this study cementum proteins were extracted from bovine and human teeth by sequential chaotropic extraction using guanidine followed by guanidine/EDTA. The guanidine/EDTA extract, but not guanidine extract, was found to promote attachment of fibroblasts. This attachment activity was inhibitable with synthetic peptide containing the attachment sequence arginine‐glycine‐aspartic acid (RGD). Fractionation of the guanidine/EDTA extract revealed several fractions with attachment activity. Immunoblot analysis demonstrated that two of these fractions contain the bone‐associated RGD containing attachment protein, bone sialoprotein‐II (BSP‐II). In addition, attachment activity was also noted in other fractions that could not be attributed to BSP‐II or fibronectin. These studies indicate that a component of the attachment activity of cementum is likely to be due to BSP‐II and that cementum contains additional, as yet undetermined, attachment proteins.