Interaction of calcium with porcine adrenal chromogranin A (secretory protein-I) and chromogranin B (secretogranin I).
Interaction of calcium with porcine adrenal chromogranin A (secretory protein-I) and chromogranin B (secretogranin I).
复制标题
钙与猪肾上腺嗜铬粒蛋白 A（分泌蛋白-I）和嗜铬粒蛋白 B（分泌蛋白 I）的相互作用。
DOI：
10.1152/ajpendo.1989.257.2.e247
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Cohn,DV
中科院分区：
文献类型：
--
作者：
Gorr,SU;Shioi,J;Cohn,DV
内分泌细胞的分泌颗粒含有一种或多种酸性分泌蛋白：嗜铬粒蛋白A（分泌蛋白I）、嗜铬粒蛋白B（分泌粒蛋白I）和嗜铬粒蛋白II（分泌粒蛋白C）。有人提出这些蛋白质在分泌产物的包装中起作用。在目前的研究中，纯化的猪肾上腺染色质颗粒的裂解物含有铬粒蛋白A和B和一个推测的铬粒蛋白B片段结合钙，并在10-20毫米钙存在下，在pH 5-6和100毫米或更少的KCl， NaCl或去甲肾上腺素下形成聚集体。沉淀物几乎包含了所有的嗜铬粒蛋白B和嗜铬粒蛋白B片段以及约三分之一的嗜铬粒蛋白a。在细胞外液的pH值和盐浓度下，聚集体不形成或被解离。钙从纯溶液中析出纯化的铬粒蛋白A和铬粒蛋白B，析出程度与从颗粒裂解物中析出的程度相同。添加到裂解物中的甲状旁腺激素被掺入沉淀物中，而酸性分泌蛋白卵清蛋白和去甲肾上腺素则没有。这些发现表明，分泌蛋白- 1和分泌颗粒蛋白可以作为聚集体存在于原位，可能包括选定的分泌产物。
Secretory granules of endocrine cells contain one or more of the acidic secretory proteins chromogranin A (secretory protein-I), chromogranin B (secretogranin I), and secretogranin II (chromogranin C). It has been proposed that these proteins play a role in the packaging of secretory products. In the present study, lysates of purified porcine adrenal chromaffin granules containing chromogranins A and B and a putative chromogranin B fragment bound calcium and formed aggregates in the presence of 10–20 mM calcium at pH 5–6 and at 100 mM or less KCl, NaCl, or norepinephrine. The precipitates contained virtually all of the chromogranin B and the chromogranin B fragment and about one-third of the chromogranin A. The aggregates did not form or were dissociated at the pH and salt concentration of the extracellular fluid. Calcium precipitated purified chromogranin A and chromogranin B from pure solution to the same extent as from the granule lysates. Parathormone, added to the lysates, was incorporated in the precipitates, whereas the acidic secretory protein ovalbumin and norepinephrine were not. These findings suggest that secretory protein-I and secretogranin can exist in situ as aggregates that may include selected secretory products.