Culture of chestnut shoots from buds in vitro.
Culture of chestnut shoots from buds in vitro.
复制标题
板栗芽的体外培养。
DOI：
10.1080/00221589.1980.11514906
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
M. L. Vieitez
中科院分区：
文献类型：
--
作者：
A. Viéitez;M. L. Vieitez
摘要本文介绍了一种从3-4月龄板栗实生苗的侧芽在营养液中培养出离体新梢的方法。BAP的培养基中，最佳的浓度为1毫克升-1引起腋芽的发展。玉米素诱导更旺盛的芽，但很少有腋芽的发展。激动素没有效果，与对照相比，没有细胞分裂素。当向培养物中加入GA 3时，观察到对生长的不利影响。在含有0.1 mg l-1 BAP的培养基中建立了从初始培养物上的芽切下的单芽和节的亚培养物，以促进进一步的芽增殖。
SummaryA method is described of growing chestnut shoots in vitro from lateral buds of 3-4 month-old seedlings cultured in a nutrient medium. The addition of BAP to the culture medium, optimally at a concentration of 1 mg l-1 caused the development of axillary shoots. Zeatin induced more vigorous shoots, but with little development of axillary shoots. Kinetin had no effect, compared to the control, without cytokinin. An adverse effect on growth was observed when GA3 was added to the cultures. Sub-cultures of single shoots and nodes excised from shoots on the initial cultures were established in a medium with 0.1 mg l-1 BAP to promote further shoot multiplication.