Timing the early events during sea urchin fertilization.
Timing the early events during sea urchin fertilization.
复制标题
确定海胆受精过程中早期事件的时间。
DOI：
10.1016/0012-1606(83)90217-8
复制
发表时间：
1983
影响因子：
2.7
通讯作者：
Hülser,D
中科院分区：
文献类型：
--
作者：
Schatten,G;Hülser,D
为了精确地确定海胆（Lytechinus variegatus）受精过程中最早事件的时间、持续时间和顺序，通过视频混合，将微电极刺穿的卵的生物电记录电子地叠加在受精时同一卵的显微差分干涉对比图像上。使用慢动作分析仪的录像分析表明，成功的精子在精卵附着的3.36±3.02秒（0.72-9.76秒范围；Σ = 23个卵子）内触发生物电膜电位反转。在12.66±2.72秒（6.72-16.60秒范围；Σ = 15）后，当精子尾部停止跳动，精子结合时，这个精子在附着部位积极旋转，与另一个不成功的精子无法区分。受精势开始后20.79±3.18秒（13.62-26.08秒；Σ = 12），即结合过程中精尾运动停止后平均约8秒，皮质颗粒开始排出，受精被膜开始在融合部位升高。在大多数情况下，生物电反应在精子粘附后7秒内开始；如果排除少数缓慢的情况，对数据进行分析，受精潜力在精子附着后1.93秒（±1.28秒）开始。这些结果表明，第一个成功的精子在精卵粘附的3.4秒（可能最快1.9秒）内触发了多精的快速阻塞，大约在受精的第一个形态学指示之前13秒，在受精被毛的特征升高之前大约21秒，导致多精的后期阻塞。
To determine precisely the timing, duration, and sequences of the earliest events during sea urchin (Lytechinus variegatus) fertilization, the bioelectric recordings of microelectrode-impaled eggs were electronically superimposed, by video mixing, over the microscopic differential interference contrast image of the same egg at insemination. Videotape analysis, utilizing a slow-motion analyzer, demonstrates that the successful sperm triggers the bioelectric membrane potential reversal within 3.36 ± 3.02 sec (0.72–9.76 sec range; Σ = 23 eggs) of sperm-egg attachment. This sperm, actively gyrating about its attachment site, is indistinguishable from the other, unsuccessful sperm until 12.66 ± 2.72 sec (6.72–16.60 sec range; Σ = 15) later when the sperm tail ceases its beating and sperm incorporation ensues. The cortical granules begin to discharge, and the fertilization coat starts to elevate at the fusion site at 20.79 ± 3.18 sec (13.62–26.08 sec range; Σ = 12) after the onset of the fertilization potential, i.e., an average of about 8 sec after the cessation of sperm-tail motility during incorporation. In most cases, the bioelectric responses starts within 7 sec of sperm adhesions; if the data are analyzed excluding the few slow cases, the fertilization potential is found to start 1.93 sec (±1.28 sec) after sperm attachment. These results indicate that the first successful sperm triggers the fast block to polyspermy within 3.4 sec, perhaps as quickly as 1.9 sec, of sperm-egg adhesion, about 13 sec before the first morphological indication of fertilization, and about 21 sec before the characteristic elevation of the fertilization coat responsible for the late block to polyspermy.