Serum miR-375-3p increase in mice exposed to a high dose of ionizing radiation.
Serum miR-375-3p increase in mice exposed to a high dose of ionizing radiation.
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暴露于高剂量电离辐射的小鼠中血清miR-375-3p增加。
DOI：
10.1038/s41598-018-19763-7
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发表时间：
2018-01-22
影响因子：
4.6
通讯作者：
Wojcik A
中科院分区：
文献类型：
--
作者：
Chiba M;Monzen S;Iwaya C;Kashiwagi Y;Yamada S;Hosokawa Y;Mariya Y;Nakamura T;Wojcik A
暴露于高剂量电离辐射（IR）会导致哺乳动物出现强急性辐射综合征（ARS）。ARS有潜伏期，因此开发快速的预后生物标志物对其早期发现和评估具有重要意义。外周血淋巴细胞染色体畸变的分析是生物剂量学的金标准，但在高剂量IR后就失效了。因此，建立在高剂量范围内快速可靠的新型暴露生物标志物是很重要的。在这里，我们研究了miRNA水平在小鼠血清中的适用性。我们发现，在全身暴露于7 Gy的x射线后，miR-375-3p的水平显著增加。此外，我们分析了它们在对照小鼠各器官中的水平，发现它们在胰腺和肠道中尤其丰富。在7 Gy剂量后，这些组织中发生了广泛的细胞死亡，这与器官中miR-375-3p的水平呈负相关。我们得出结论，高表达的miR-375-3p可能在暴露于7 Gy后在血清中分泌这种miRNA。因此，血清中miR-375-3p升高可能是暴露于高剂量辐射引起的组织损伤的预测因子。
Exposure to high-doses of ionizing radiation (IR) leads to development of a strong acute radiation syndrome (ARS) in mammals. ARS manifests after a latency period and it is important to develop fast prognostic biomarkers for its early detection and assessment. Analysis of chromosomal aberrations in peripheral blood lymphocytes is the gold standard of biological dosimetry, but it fails after high doses of IR. Therefore, it is important to establish novel biomarkers of exposure that are fast and reliable also in the high dose range. Here, we investigated the applicability of miRNA levels in mouse serum. We found significantly increased levels of miR-375-3p following whole body exposure to 7 Gy of X-rays. In addition, we analyzed their levels in various organs of control mice and found them to be especially abundant in the pancreas and the intestine. Following a dose of 7 Gy, extensive cell death occurred in these tissues and this correlated negatively with the levels of miR-375-3p in the organs. We conclude that high expressing tissues of miR-375-3p may secrete this miRNA in serum following exposure to 7 Gy. Therefore, elevated miR-375-3p in serum may be a predictor of tissue damage induced by exposure to a high radiation dose.
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影响因子：
5.6
作者：
Mirzayans R;Andrais B;Scott A;Wang YW;Murray D
通讯作者：
Murray D
影响因子：
4.8
作者：
Erener, Suheda;Mojibian, Majid;Kieffer, Timothy J.
通讯作者：
Kieffer, Timothy J.
影响因子：
3.7
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Glinge C;Clauss S;Boddum K;Jabbari R;Jabbari J;Risgaard B;Tomsits P;Hildebrand B;Kääb S;Wakili R;Jespersen T;Tfelt-Hansen J
通讯作者：
Tfelt-Hansen J
影响因子：
3.7
作者：
Ho YK;Xu WT;Too HP
通讯作者：
Too HP
影响因子：
64.8
作者：
Denli, AM;Tops, BBJ;Hannon, GJ
通讯作者：
Hannon, GJ