Aspects of the male reproductive toxicity/male antifertility property of andrographolide in albino rats: Effect on the testis and the cauda epididymidal spermatozoa
Aspects of the male reproductive toxicity/male antifertility property of andrographolide in albino rats: Effect on the testis and the cauda epididymidal spermatozoa
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DOI：
10.1002/1099-1573(200009)14:6
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发表时间：
2000-09-01
影响因子：
7.2
通讯作者：
Murugaian, P
中科院分区：
文献类型：
--
作者：
Akbarsha, MA;Murugaian, P
先前的研究表明，当给雄性白化病大鼠喂食穿心莲叶时，会导致精子发生停滞。本研究旨在探讨穿心莲的主要成分穿心莲内酯是否具有这种作用。将穿心莲内酯以两个剂量水平给予5月龄雄性Wistar白化病大鼠，连续48天，进行生育试验，附睾尾精子计数、活动力和异常分析，以及睾丸组织病理学评价。结果表明，精子数量减少，精子不活动，部分精子有异常。生精上皮被彻底破坏，在生精小管中，完全分化的精子非常有限;处于分裂阶段的细胞很普遍;多核巨细胞丰富，间质细胞似乎完好无损。推测穿心莲内酯可能通过抑制分裂期生精细胞系的胞质分裂而影响精子发生。多核巨细胞与细胞松弛素-D和熊果酸产生的共质体相当，这是由于作用于生精周期的V-VII阶段，支持细胞损伤和精子毒性作用也很明显。因此，该研究指出了该化合物作为治疗药物时的男性生殖毒性作用;该研究还证实了穿心莲内酯在男性避孕中的潜在用途。版权所有（C）2000约翰威利父子有限公司
Previous work has shown that Andrographis paniculata leaf, when fed to male albino rats, causes the arrest of spermatogenesis. The present study was undertaken to investigate whether andrographolide, one of the major constituents of this plant, is responsible for such an effect, The compound was administered to S-month-old male Wistar albino rats at two dose levels, for 48 days, Fertility tests, analysis of the counts, motility and abnormalities of the cauda epididymidal spermatozoa, and histopathological evaluation of the testis were carried out. The results showed that sperm counts decreased, the spermatozoa were not motile, and several of them possessed abnormalities. The seminiferous epithelium was thoroughly disrupted and in the seminiferous tubules, fully differentiated spermatozoa were far too limited; cells in the divisional stages were prevalent; multinucleate giant cells were abundant and Leydig cells appeared intact. It is inferred that andrographolide could affect spermatogenesis by preventing cytokinesis of the dividing spermatogenic cell lines. The multinucleate giant cells are comparable to the symplasts generated by cytochalasin-D and ursolic acid due to action at stages V-VII of the spermatogenic cycle, Sertoli cell damage and spermatotoxic effects are also apparent. Thus, the study points to a male reproductive toxic effect of this compound when used as a therapeutic; the study also confirms the possible prospective use of andrographolide in male contraception. Copyright (C) 2000 John Wiley & Sons, Ltd.