One-step separation of lysozyme by reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide
One-step separation of lysozyme by reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide
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DOI：
10.1016/j.foodchem.2004.09.012
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发表时间：
2005-11
期刊：
影响因子：
8.8
通讯作者：
Kyung-Hyun Noh;J. Imm
中科院分区：
文献类型：
--
作者：
Kyung-Hyun Noh;J. Imm
用阳离子表面活性剂十六烷基二甲基溴化铵（CDAB）形成的反胶束从复溶冻干蛋清中选择性提取溶菌酶。主要的蛋清蛋白，包括卵清蛋白和卵转铁蛋白，被溶解到有机相，而溶菌酶在水相回收。蛋白质的增溶行为通过处理参数来操纵，包括水相中的pH和盐浓度以及有机相中的表面活性剂浓度。使用硼酸钠缓冲液（50 mM，pH 9，不添加KCl）和含有50 mM CDAB的有机相实现最佳提取。正萃取后，溶菌酶总活力回收率为96%。溶菌酶被有效地纯化，超过30倍，只有一个单一的正向提取。与传统的需要前向和后向萃取步骤的反胶束萃取相比，所建议的萃取程序在时间和成本方面具有优势。
Lysozyme was selectively extracted from reconstituted freeze-dried egg-white, using reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide (CDAB). The major egg-white proteins, including ovalbumin and ovotansferrin, were solubilized into the organic phase while lysozyme was recovered in the aqueous phase. The solubilization behaviours of proteins were manipulated by processing parameters, including pH and salt concentration in the aqueous phase and concentration of surfactant in the organic phase. The optimum extraction was achieved with sodium borate buffer (50 mM, pH 9, no added KCl) and organic phase containing 50 mM CDAB. After the forward extraction, 96% of total lysozyme activity was recovered. Lysozyme was efficiently purified, more than 30-fold with only a single forward extraction. The suggested extraction procedure has advantages in terms of time and cost compared to traditional reverse micellar extraction which requires both forward and backward extraction steps.