Human β-defensin-2 as a marker for disease severity and skin barrier properties in atopic dermatitis
Human β-defensin-2 as a marker for disease severity and skin barrier properties in atopic dermatitis
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DOI：
10.1111/bjd.12419
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发表时间：
2013-09-01
影响因子：
10.3
通讯作者：
Agner, T.
中科院分区：
文献类型：
--
作者：
Clausen, M. -L.;Jungersted, J. M.;Agner, T.
背景 与抗菌防御破坏相关的皮肤感染是特应性皮炎 (AD) 的常见问题。据报道，AD 皮肤中抗菌肽（包括人防御素 (hBD)-2）的水平发生了变化，并表明与屏障功能受损有关。 目的 研究 hBD-2 与 AD 患者和对照者皮肤屏障功能的关系，以及研究 hBD-2 与疾病严重程度的关系。 方法 纳入 25 名 AD 患者和 11 名对照者。通过微创胶带剥离法收集角质层样品，测定 hBD-2 肽浓度。通过特应性皮炎评分 (SCORAD) 评估疾病严重程度，并通过测量经表皮失水 (TEWL) 和皮肤 pH 值评估皮肤屏障功能。根据丝聚蛋白突变对 AD 患者进行表征。结果发现，病变和非病变 AD 皮肤以及对照者角质层中的 hBD-2 浓度存在差异，其中病变皮肤的浓度最高（P < 0.001）。在可测量 hBD-2 的参与者中，SCORAD 和 TEWL 显着增加（分别为 P < 0.018 和 P < 0.007）。观察到病变皮肤中的 hBD-2 与 TEWL 和 SCORAD 之间存在显着相关性（分别为 R = 0.55 和 R = 0.44）。没有发现与皮肤 pH 值的相关性。未发现 hBD-2 与聚丝蛋白突变相关。结论 hBD-2、皮肤屏障功能紊乱和疾病严重程度之间存在显着相关性。微创皮肤样本技术能够随着时间的推移评估角质层及其蛋白质，并提供将这些发现与治疗、感染和生理变化联系起来的可能性。
Background Skin infections related to disrupted antimicrobial defence are a common problem in atopic dermatitis (AD). Altered levels of antimicrobial peptides, including human -defensin (hBD)-2, have been reported in AD skin, and a link to impaired barrier function has been suggested.Objectives To study hBD-2 in relation to skin barrier function in patients with AD and controls, and to study hBD-2 in relation to disease severity.Methods Twenty-five patients with AD and 11 controls were enrolled. hBD-2 peptide concentration was determined in stratum corneum samples collected by a minimally invasive tape-stripping method. Disease severity was assessed by SCORing Atopic Dermatitis (SCORAD), and skin barrier function was evaluated by measurement of transepidermal water loss (TEWL) and skin pH. Patients with AD were characterized according to filaggrin mutations.Results hBD-2 concentrations in the stratum corneum were found to differ between lesional and nonlesional AD skin and controls, with the highest values in lesional skin (P < 0.001). SCORAD and TEWL were significantly increased in participants with measureable hBD-2 (P < 0.018 and P < 0.007, respectively). Significant correlations between hBD-2 in lesional skin, and TEWL and SCORAD were observed (R = 0.55 and R = 0.44, respectively). No correlations with skin pH were found. hBD-2 was not found to relate to filaggrin mutations.Conclusions A significant correlation was found between hBD-2, disturbed skin barrier function and disease severity. The minimally invasive skin sample technique enables evaluation of the stratum corneum and its proteins over time and provides the possibility of relating these findings to treatment, infections and physiological variations.