Proliferation and osmotic tolerance of renal inner medullary epithelial cells in vivo and in cell culture.
Proliferation and osmotic tolerance of renal inner medullary epithelial cells in vivo and in cell culture.
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体内和细胞培养中肾内髓质上皮细胞的增殖和渗透耐受性。
DOI：
10.1152/ajprenal.00038.2002
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Burg,MauriceB
中科院分区：
文献类型：
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作者：
Zhang,Zheng;Cai,Qi;Michea,Luis;Dmitrieva,NataliaI;Andrews,Peter;Burg,MauriceB
肾内髓（IM）细胞在间质渗透压为600 - 1，700 mosmol/kgH 2 O或更高时存活。相比之下，更小的急性变化杀死了以前在组织培养中研究的细胞，例如小鼠IM集合管3（mIMCD 3）细胞，它们用SV 40永生化并迅速增殖。增殖和DNA复制使mIMCD 3细胞对高渗敏感。在目前的研究中，我们观察到，增殖细胞在大鼠IM稀缺。然后，我们将第2代小鼠IM上皮细胞（p2 mIME）进行传代。它们的DNA复制发生率比mIMCD 3细胞低得多。p2 mIME细胞比mIMCD 3细胞在NaCl的急性增加中存活得更大，并且还耐受尿素和NaCl加尿素的显著更大的急性增加，但仍然没有达到体内发生的高水平。我们的结论是，永生化和持续的DNA复制帐户的一部分，以前观察到的IM细胞在体内和细胞培养之间的渗透耐受性的差异，但其他因素也必须参与。
Renal inner medullary (IM) cells survive interstitial osmolality that ranges from 600 to 1,700 mosmol/kgH2O or more. In contrast, much smaller acute changes killed the cells previously studied in tissue culture, such as mouse IM collecting duct 3 (mIMCD3) cells, that are immortalized with SV40 and proliferate rapidly. Proliferation and DNA replication sensitize mIMCD3 cells to hypertonicity. In the present studies, we observed that proliferating cells were scarce in rat IM. Then, we preparedpassage 2mouse IM epithelial (p2mIME) cells. They have a much lower incidence of DNA replication than do mIMCD3 cells. p2mIME cells survive much greater acute increases in NaCl than do mIMCD3 cells and also tolerate significantly greater acute increases of urea and of NaCl plus urea, but still not to levels as high as occur in vivo. We conclude that immortalization and continued DNA replication account for part of the previously observed difference in osmotic tolerance between IM cells in vivo and in cell culture but that other factors must also be involved.