Expression of peroxiredoxins in bovine oocytes and embryos produced in vitro
Expression of peroxiredoxins in bovine oocytes and embryos produced in vitro
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DOI：
10.1002/mrd.20145
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发表时间：
2004-11-01
影响因子：
2.5
通讯作者：
Donnay, I
中科院分区：
文献类型：
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作者：
Leyens, G;Knoops, B;Donnay, I
过氧化物还原酶（PRDXs）是参与抗氧化保护和细胞信号传导的过氧化物酶家族。由于它们的过氧化物还原酶活性，这些酶可能参与在体外生产过程中微调胚胎中的过氧化物水平。本研究采用RT-PCR技术检测了PRDX 6种亚型（PRDX1~PRDX6）在牛卵母细胞和胚胎中的表达。在卵母细胞体外成熟前后均检测到PRDXs。此外，PRDX6在成熟后表达上调。从两细胞到囊胚阶段分析单个胚胎。在整个开发过程中检测到PRDX1和PRDX5转录本。PRDX2、PRDX3和PRDX6在9 - 16细胞期前后不表达。从9 - 16-细胞期开始，在胚胎池中检测到PRDX4转录本。PRDX5的原位免疫检测，这是以前报道的成年哺乳动物细胞中的PRDXs表现出最广泛的亚细胞分布，在牛胚胎中的线粒体分布模式。最后，通过在20%O-2而不是5%下培养胚胎来评估主要胚胎基因组激活周围PRDX表达的氧化应激的潜在调节。在20%O-2条件下，PRDX表达模式无显著差异。总之，我们的数据首次表明PRDXs在哺乳动物卵母细胞和早期胚胎中表达。此外，牛的成绩单表现出各种模式的表达，可能与PRDXs在卵母细胞成熟和胚胎发育的潜在作用。(C)2004威利-利斯公司
Peroxiredoxins (PRDXs) form a family of peroxidases involved in antioxidant protection and cell signaling. Due to their peroxide reductase activity, these enzymes might be involved in fine-tuning peroxide levels in embryos during in vitro production. In this study, RT-PCR was used to examine the expression of the six PRDX isoforms (PRDX1 to PRDX6) in bovine oocytes and embryos. PRDXs were detected in oocytes both before and after in vitro maturation. Besides, PRDX6 was up-regulated after maturation. Single embryos were analyzed from the two-cell to the blastocyst stages. PRDX1 and PRDX5 transcripts were detected throughout development. PRDX2, PRDX3, and PRDX6 were not expressed around the 9- to 16-cell stage. PRDX4 transcripts were weakly detected in pools of embryos from the 9- to 16-cell stage onwards. In situ immunodetection of PRDX5, which was previously reported to exhibit the widest subcellular distribution among PRDXs in adult mammalian cells, showed a mitochondrial distribution pattern in the bovine embryo. Finally, the potential modulation by oxidative stress of PRDX expression around the major embryonic genome activation was evaluated by culturing embryos under 20% O-2 instead of 5%. No significant difference in the pattern of PRDX expression was observed under 20% O-2. In conclusion, our data show for the first time that PRDXs are expressed in mammalian oocytes and early embryos. Moreover, the bovine transcripts exhibit various patterns of expression that might be related to the potential role of PRDXs in oocyte maturation and embryo development. (C) 2004 Wiley-Liss, Inc.