In vitro potentiation of human natural killer cell activity by a streptococcal preparation, OK-432: interferon and interleukin-2 participation in the stimulation with OK-432.
In vitro potentiation of human natural killer cell activity by a streptococcal preparation, OK-432: interferon and interleukin-2 participation in the stimulation with OK-432.
复制标题
链球菌制剂 OK-432 体外增强人自然杀伤细胞活性：干扰素和白细胞介素 2 参与 OK-432 的刺激。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
Y. Morioka
中科院分区：
文献类型：
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作者：
H. Wakasugi;T. Kasahara;N. Minato;J. Hamuro;M. Miyata;Y. Morioka
由于链球菌免疫增强剂OK-432在体内和体外均显著增强人自然杀伤（NK）细胞活性，因此分析了OK-432增强人NK细胞活性的机制。OK-432刺激的非贴壁淋巴细胞的培养上清液显著增强NK细胞活性。干扰素（IFN）（α-和γ-型）和白细胞介素-2（IL-2）的显着活性中检测到的非粘附淋巴细胞的培养上清液。用抗IFN-α抗血清和pH-2甘氨酸-HCl缓冲液同时处理上清液或用IL-2依赖性细胞系吸收上清液完全消除了NK增强活性，而用这些中的任一种处理仅导致活性的部分消除。这些结果表明，OK-432刺激人非粘附淋巴细胞产生IFN和IL-2，这两个因素是主要负责的NK增强OK-432。
Inasmuch as human natural killer (NK) cell activity was markedly augmented by a streptococcal immunopotentiator, OK-432, both in vivo and in vitro, the mechanism in which OK-432 augmented human NK cell activity was analyzed. Culture supernatants of nonadherent lymphocytes stimulated with OK-432 significantly augmented NK cell activity. Significant activity of both interferon (IFN) (both alpha- and gamma-types) and interleukin-2 (IL-2) was detected in the culture supernatants from nonadherent lymphocytes. Concomitant treatment of supernatants with anti-IFN-alpha antiserum and pH-2 glycine-HCI buffer or the absorption of supernatants with an IL-2-dependent cell line completely abrogated the NK-augmenting activity, whereas the treatment with either one of these resulted in only partial elimination of the activity. These results indicate that OK-432 stimulates human nonadherent lymphocytes to produce IFN and IL-2 and that both factors are primarily responsible for the NK augmentation by OK-432.