Macroporous ordered titanium dioxide (TiO2) inverse opal as a new label-free immunosensor
Macroporous ordered titanium dioxide (TiO2) inverse opal as a new label-free immunosensor
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DOI：
10.1016/j.aca.2008.07.008
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发表时间：
2008-09-05
影响因子：
6.2
通讯作者：
Lu, Zuhong
中科院分区：
文献类型：
--
作者：
Li, Jianlin;Zhao, Xiangwei;Lu, Zuhong
光子晶体传感材料对折射率变化非常敏感。本文采用自组装技术制备了三维有序大孔（3DOM） （bbb50 nm） TiO2反蛋白石膜。基于TiO2反蛋白石膜，建立了无标记免疫传感器分光计。采用人IgG/山羊抗人IgG对3DOM 7102进行检测，结果表明3DOM TiO2反蛋白石膜的灵敏度可达1 μ g mL(-1)（相当于1.5 pg mm(-2)）的蛋白浓度检测限。3DOM TiO2反蛋白石具有较大的内表面积、低荧光背景和独特的光学性质。这些特性表明3DOM TiO2逆蛋白石在无标记免疫分析中的可行性。(c) 2008 Elsevier B.V.版权所有
Photonic crystal sensing materials have been validated that they are very sensitive to refractive index changes. Herein, three-dimensionally ordered macroporous (3DOM) (> 50 nm) TiO2 inverse opal film has been fabricated by the self-assembly technique. Based on the TiO2 inverse opal film, the optical spectrometer was established for label-free immunosensor. The sensing performance of the 3DOM 7102 was investigated using human IgG/goat antihuman IgG couple, which showed that the sensitivity of 3DOM TiO2 inverse opal film could reach to 1 mu g mL(-1) (equivalent to 1.5 pg mm(-2)) of protein concentration detection limit. The 3DOM TiO2 inverse opal has a large internal surface area, low fluorescence background and unique optical properties. These characteristics indicated the feasibility of 3DOM TiO2 inverse opal in label-free immunoassay. (c) 2008 Elsevier B.V. All rights reserved.