Volatile compounds emitted by sclerotia of Sclerotinia minor, Sclerotinia sclerotiorum, and Sclerotium rolfsii.

Volatile compounds emitted by sclerotia of Sclerotinia minor, Sclerotinia sclerotiorum, and Sclerotium rolfsii.
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小核盘菌、核盘菌和罗氏菌核菌的菌核散发出的挥发性化合物。

DOI:
10.1021/jf0117098
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发表时间:
2002
影响因子:
6.1
通讯作者:
S. Lloyd
S. Lloyd
中科院分区:
农林科学1区
文献类型:
--
作者:
D. Fravel;W. Connick;C. Grimm;S. Lloyd

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采用固相微萃取、气相色谱和质谱技术对小菌核菌(Sclerotinia minor)、菌核菌(Sclerotinia sclerotiorum)和罗氏菌核菌(Sclerotinia rolfsii)的挥发物进行了鉴定。小松茸和菌核松茸均释放2-甲基冰片烯和2-甲基异冰片烯。此外,S. minor还释放出二甲氧基氧化物、γ -丁内酯、顺式和反式芳樟醇氧化物、芳樟醇和反式神经樟醇。菌丝体释放出2-甲基-2-冰片烯、1-甲基莰烯和一种分子量为272的二萜。罗尔夫核菌核不释放这些化合物中的任何一种,但却释放出-二硝基二烯和顺式卡拉烯。研究人员对小葡萄球菌和菌核葡萄球菌释放的化学物质进行了测试,以确定它们是否能刺激菌核菌菌核上的一种分枝寄生虫孢子菌菌核菌孢子的萌发。化学物质在十亿分之一到百万分之一的浓度下进行了测试,包括直接接触孢子和近距离接触(但不是物理接触)。单独施用或混合施用均不能诱导菌核孢子萌发。
Volatile compounds emitted by sclerotia of Sclerotinia minor, Sclerotinia sclerotiorum, and Sclerotium rolfsii were identified by solid phase microextraction followed by gas chromatography and mass spectometry. Both S. minor and S. sclerotiorum emitted 2-methylenebornane and 2-methylisoborneol. In addition, S. minor emitted mesityl oxide, gamma-butyrolactone, cis- and trans-linalool oxide, linalool, and trans-nerolidol. S. sclerotiorum emitted 2-methyl-2-bornene, 1-methylcamphene, and a diterpene with a molecular weight of 272. Sclerotium rolfsii did not emit any of these compounds but did emit delta-cadinene and cis-calamenene. Chemicals emitted by S. minor and S. sclerotiorum were tested to determine if they could stimulate germination of conidia of Sporidesmium sclerotivorum, a mycoparasite on sclerotia of Sclerotinia spp. Chemicals were tested at 1 part per billion to 100 parts per million, both in direct contact with conidia and near, but not in, physical contact. None of the chemicals alone nor a combination of all chemicals induced germination of conidia of S. sclerotivorum.