Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene
Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene
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DOI：
10.1073/pnas.0604698103
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发表时间：
2006-09-26
影响因子：
11.1
通讯作者：
Hussey, Richard S.
中科院分区：
文献类型：
--
作者：
Huang, Guozhong;Allen, Rex;Hussey, Richard S.
由食道腺细胞中表达的寄生基因编码的分泌性寄生蛋白介导根结线虫（RKN）对植物的感染和寄生。寄生基因16D10编码一个保守的RKN分泌肽，它刺激根的生长，并作为一个假定的植物转录因子的配体发挥作用。本研究利用RNA干扰技术，在RKN中沉默了该寄生基因，证实了该寄生基因在RKN的寄生中具有重要的功能。在体外摄入16D10 dsRNA沉默RKN中的靶寄生基因，并导致线虫感染性降低。拟南芥中1161310 dsRNA的体内表达导致对四种主要RKN物种有效的抗性。由于没有已知的天然抗性基因具有如此广泛的RKN抗性有效范围，表达沉默靶向RKN寄生基因以破坏寄生过程的dsRNA的生物工程作物代表了开发新型持久的RKN抗性作物的可行且灵活的手段，并且可以为作物提供对RKN的前所未有的广泛抗性。
Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence this parasitism gene in RKN and validate that the parasitism gene has an essential function in RKN parasitism of plants. Ingestion of 16D10 dsRNA in vitro silenced the target parasitism gene in RKN and resulted in reduced nematode infectivity. In vivo expression of 1161310 dsRNA in Arabidopsis resulted in resistance effective against the four major RKN species. Because no known natural resistance gene has this wide effective range of RKN resistance, bioengineering crops expressing dsRNA that silence target RKN parasitism genes to disrupt the parasitic process represents a viable and flexible means of developing novel durable RKN-resistant crops and could provide crops with unprecedented broad resistance to RKN.