Theoretical and experimental studies on cross-bridge migration during cell disaggregation.
Theoretical and experimental studies on cross-bridge migration during cell disaggregation.
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DOI：
10.1016/s0006-3495(89)82841-3
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发表时间：
1989-03
影响因子：
3.4
通讯作者：
A. Tozeren;K. Sung;S. Chien
中科院分区：
文献类型：
--
作者：
A. Tozeren;K. Sung;S. Chien
采用显微操作方法测定细胞毒性T细胞对（F1）与其靶细胞（JY: HLA-A2-B7-DR4,W6）之间的粘附能量密度（gamma）。伽马被定义为必须提供的每单位面积的能量，以减少共轭细胞对之间的接触区域。我们对数据的分析表明，微移液管施加在细胞上的力并不像我们之前假设的那样均匀分布在整个接触区域（Sung, k.l.p., l.a. Sung， M. Crimmins， S. J. Burakoff, and S. Chien. 1986）。科学(洗。直流)。234: 1405-1408)，但只在接触区域的边缘起作用。我们发现，在剥离过程中伽马不是恒定的，而是随着共轭细胞对F1-JY， F1-F1和JY-JY的接触面积的减少而增加，与典型工程粘合剂的伽马常数相反。这一发现支持了这样一种观点，即交联蛋白分子沿着分离的前沿向共轭区域滑动，同时仍附着在两个细胞上。我们的数学分析表明，膜张力储存在交联中的弹性能量平衡了阻止跨桥迁移的扩散力。发现F1-JY之间的结合亲和力大约是F1-F1对应亲和力的15-20倍。F1与JY的结合位点数量与F1与另一个F1的结合位点数量大致相同，在10(5)到10(6)之间变化。
A micromanipulation method is used to determine the adhesive energy density (gamma) between pairs of cytotoxic T cells (F1) and their target cells (JY: HLA-A2-B7-DR4,W6). gamma is defined as the energy per unit area that must be supplied to reduce the region of contact between a conjugated cell pair. Our analysis of the data indicates that the force applied by the micropipette on the cell is not uniformly distributed throughout the contact region as we had previously assumed (Sung, K. L. P., L. A. Sung, M. Crimmins, S. J. Burakoff, and S. Chien. 1986. Science (Wash. DC). 234: 1405–1408), but acts only at the edges of the contact region. We show that gamma is not constant during peeling but increases with decreasing contact area of the conjugated cell pairs F1-JY, F1-F1, and JY-JY in contrast to the constancy of gamma for typical engineering adhesives. This finding supports the notion that the cross-linking protein molecules slide towards the conjugated area across the leading edge of the separation while remaining attached to both cells. Our mathematical analysis shows that the elastic energy stored in the cross-links by the membrane tensions balances the diffusive forces that act against cross-bridge migration. The binding affinity between F1-JY is found to be approximately 15–20 times larger than the corresponding affinity for F1-F1. The number of binding sites of F1 for attachment to JY is approximately the same for binding F1 to another F1 and vary between 10(5) and 10(6).