Sweat lipid mediator profiling: a noninvasive approach for cutaneous research
Sweat lipid mediator profiling: a noninvasive approach for cutaneous research
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DOI：
10.1194/jlr.m071738
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发表时间：
2017-01-01
影响因子：
6.5
通讯作者：
Newman, John W.
中科院分区：
文献类型：
--
作者：
Agrawal, Karan;Hassoun, Lauren A.;Newman, John W.
分析和汗液收集技术的最新进展为识别用于皮肤炎症和修复研究的非侵入性生物标志物提供了新的机会。本研究的目的是表征脂质介质的配置文件，包括含氧脂质，内源性大麻素，和神经酰胺/鞘氨醇碱在汗液中，并确定这些配置文件之间的差异汗液收集从非病变部位上的unflared掌侧前臂的受试者和没有特应性皮炎（AD）。调整常规程序开发的血浆分析，超过100个脂质介质，使用LC-MS/MS和58个脂质介质检测汗液。脂质介质浓度不受采样或储存条件的影响。在患有AD的研究参与者的汗液中观察到C30-C40 [NS]和[NdS]神经酰胺以及C18：1鞘氨醇的浓度增加，尽管在研究组之间没有观察到经表皮水分损失的差异，并且这种作用在男性中最强（P < 0.05，具有Tukey事后HSD的单因素ANOVA）。在研究组之间没有观察到氧脂素和内源性大麻素的差异。(Jlr)因此，汗液介质分析可以在出现临床体征之前为AD提供非侵入性诊断。
Recent advances in analytical and sweat collection techniques provide new opportunities to identify noninvasive biomarkers for the study of skin inflammation and repair. This study aims to characterize the lipid mediator profile including oxygenated lipids, endocannabinoids, and ceramides/sphingoid bases in sweat and identify differences in these profiles between sweat collected from nonlesional sites on the unflared volar forearm of subjects with and without atopic dermatitis (AD). Adapting routine procedures developed for plasma analysis, over 100 lipid mediators were profiled using LC-MS/MS and 58 lipid mediators were detected in sweat. Lipid mediator concentrations were not affected by sampling or storage conditions. Increases in concentrations of C30-C40 [NS] and [NdS] ceramides, and C18:1 sphingosine, were observed in the sweat of study participants with AD despite no differences being observed in transepidermal water loss between study groups, and this effect was strongest in men (P < 0.05, one-way ANOVA with Tukey's post hoc HSD). No differences in oxylipins and endocannabinoids were observed between study groups.(Jlr) Sweat mediator profiling may therefore provide a noninvasive diagnostic for AD prior to the presentation of clinical signs.