Formation and Growth of Anionic Vesicles Followed by Small-Angle Neutron Scattering
Formation and Growth of Anionic Vesicles Followed by Small-Angle Neutron Scattering
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DOI：
10.1021/la0208732
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发表时间：
2003-04
期刊：
影响因子：
3.9
通讯作者：
I. Grillo;†‡ and E. I. Kats;A. Muratov
中科院分区：
文献类型：
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作者：
I. Grillo;†‡ and E. I. Kats;A. Muratov
我们提出了第一个动力学小角中子散射实验上进行的仪器D22（劳厄Langevin研究所，格勒诺布尔）与停流装置（生物逻辑公司）。D22为实时实验提供了独特的功能。高通量和大的q值范围仅涵盖一个仪器配置与快速电子学相结合，开辟了数百毫秒分辨率测量的可能性。我们已经使用这些技术的发展，研究自发囊泡的形成和生长后，在胶束溶液中的AOT在D2O从500毫秒至5小时混合后，添加盐（NaCl，NaBr，KCl和KBr）。囊泡半径和生长速率取决于盐浓度，并随着离子强度的增加而减小。转变的驱动力是屏蔽相邻表面活性剂头基之间的静电排斥，有利于形成局部平面双层。假设聚集是由胶束扩散控制的，一个系统。
We present the first kinetic small-angle neutron-scattering experiments carried out on the instrument D22 (Institut Laue Langevin, Grenoble) with a stopped-flow apparatus (Bio-Logic company). D22 offers unique features for real-time experiments. The high flux and the large q range covered in only one instrumental configuration combined with the rapid electronics open up possibilities for few hundred millisecond resolution measurements. We have used these technical developments to study the formation and growth of spontaneous vesicles after addition of salts (NaCl, NaBr, KCl, and KBr) in a micellar solution of AOT in D2O from 500 ms to 5 h after mixing. The vesicle radii and the growth rate depend on the salt concentration and decrease with increasing ionic strength. The driving force of the transition is the screening of the electrostatic repulsion between adjacent surfactant headgroups that favors formation of a locally planar bilayer. Assuming that the aggregation is controlled by micelle diffusion, a s...