CLEC2A: a novel, alternatively spliced and skin-associated member of the NKC-encoded AICL–CD69–LLT1 family
CLEC2A: a novel, alternatively spliced and skin-associated member of the NKC-encoded AICL–CD69–LLT1 family
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CLEC2A：NKC 编码的 AICL–CD69–LLT1 家族中的一种新型、选择性剪接且与皮肤相关的成员
作者：
Jessica Spreu;Eike Kienle;B. Schrage;A. Steinle
人类自然杀伤基因复合体(NKC)编码多种C型凝集素样受体(CTLR)，表达于多种免疫细胞，包括自然杀伤细胞(NK细胞)和髓系细胞。某些激活诱导的NKC编码的CTLR被归入C型凝集素结构域家族2(CLEC2家族)，在人类中，该家族包括AICL(CLEC2B)、CD69(CLEC2C)和LLT1(CLEC2D)。在这篇文章中，我们描述了CLEC2家族的一个新成员，人类孤儿基因CLEC2A。CLEC2A的C型凝集素样域(CTLD)与LLT1的CTLD最为相似(~60%)。与小鼠CLEC2家族成员Clr-b和Clr-g一样，CLEC2A缺乏两个高度保守的半胱氨酸(Cys4和Cys5)，这两个半胱氨酸在大多数CTLR的CTLD中形成了分子内键。外显子2和两个不同的末端外显子(外显子5A/B)的选择性剪接分别产生了四个CLEC2A变体，它们在CTLD的跨膜区和/或羧基末端部分的用途不同。CLEC2A转录本主要在髓系细胞系中检测到，但在上皮细胞系中未检测到。在组织中，CLEC2A选择性地在皮肤中表达，在较低丰度的造血和性腺组织中表达。最后，我们发现CLEC2A1变异体很容易在细胞表面表达，在那里它可能作为NKC编码的NK受体的配体。
The human natural killer gene complex (NKC) encodes for numerous C-type lectin-like receptors (CTLR), which are expressed on various immune cells including natural killer (NK) cells and myeloid cells. Certain activation-induced, NKC-encoded CTLR are grouped into the C-type lectin domain family 2 (CLEC2 family) which, in humans, comprises AICL (CLEC2B), CD69 (CLEC2C), and LLT1 (CLEC2D). In this paper, we characterize a novel member of the CLEC2 family, the human orphan gene CLEC2A. The C-type lectin-like domain (CTLD) of CLEC2A is most similar to the CTLD of LLT1 (~60% similarity). Like mouse CLEC2 family members Clr-b and Clr-g, CLEC2A lacks two highly conserved cysteines (Cys4 and Cys5), which form an intramolecular bond in the CTLD of most CTLR. Alternative splicing of exon 2 and of two distinct terminal exons (exon 5A/B), respectively, gives rise to four CLEC2A variants differing in the usage of the transmembrane domain and/or in the carboxyterminal portion of the CTLD. CLEC2A transcripts were detected primarily in myeloid cell lines, but not in epithelial cell lines. In tissues, CLEC2A is selectively expressed in the skin and, at lower abundance, in hematopoietic and gonadal tissues. Finally, we show that the CLEC2A1 variant is readily expressed at the cell surface, where it may serve as a ligand for NKC-encoded NK receptors.
影响因子：
30.5
作者：
Welte, Stefan;Kuttruff, Sabrina;Steinle, Alexander
通讯作者：
Steinle, Alexander
基于 GaN 量子阱的 249 nm 深紫外激光器
影响因子：
7
作者：
Shan, Maocheng;Zhang, Yi;Li, Xiaohang
通讯作者：
Li, Xiaohang