REAL-TIME MONITORING OF ENDOGENOUS NORADRENALINE RELEASE IN RAT-BRAIN SLICES USING FAST CYCLIC VOLTAMMETRY .1. CHARACTERIZATION OF EVOKED NORADRENALINE EFFLUX AND UPTAKE FROM NERVE-TERMINALS IN THE BED NUCLEUS OF STRIA TERMINALIS, PARS VENTRALIS
REAL-TIME MONITORING OF ENDOGENOUS NORADRENALINE RELEASE IN RAT-BRAIN SLICES USING FAST CYCLIC VOLTAMMETRY .1. CHARACTERIZATION OF EVOKED NORADRENALINE EFFLUX AND UPTAKE FROM NERVE-TERMINALS IN THE BED NUCLEUS OF STRIA TERMINALIS, PARS VENTRALIS
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DOI：
10.1016/0006-8993(92)91438-k
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发表时间：
1992-07-31
期刊：
影响因子：
2.9
通讯作者：
STAMFORD, JA
中科院分区：
文献类型：
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作者：
PALIJ, P;STAMFORD, JA
采用碳纤维微电极快速循环伏安法（FCV）实时监测腹侧终纹（BSTV）床核超薄片内源性去甲肾上腺素（NA）外排。双极钨电极局部电刺激诱发NA外排，经解剖、电化学和药理学证实释放物为NA。首先，该信号与NA神经支配密度相符：BSTV的单胺外排大于核背部。其次，释放种的伏安图与儿茶酚胺NA和多巴胺（DA）难以区分，但与吲哚胺5-羟色胺（5-羟色胺，5-HT）不同。第三，胺外排以可预测的方式受到测试药物的影响。河豚毒素（10(-6)M）和Ca2+的缺失分别可逆地减少了90.2%和88.0%的胺外排。Ro 4-1284 （10(-6) M）降低了75.8%的胺外排。选择性NA摄取阻滞剂地西帕明（5 × 10(-8) M）显著增加了胺外排和摄取半衰期（分别为对照组的214.3和389.5%）。氟伏沙明（5 × 10(-7) M）和GBR 12909 （3 × 10(-7) M）分别是5- ht和DA摄取阻滞剂，对胺外排没有影响，尽管氟伏沙明引起摄取半衰期适度增加（91.0%）。Pargyline （2 × 10(-6) M）既不影响外排也不影响吸收。结合解剖、电化学和药理学数据证实，局部电刺激检测到的BSTV单胺为NA。受刺激的NA外排在至少2.5小时（测试的最长时间）内是稳定和可重复的。该研究证明了FCV能够选择性地监测内源性NA的“实时”和高空间分辨率的外排和摄取。
Fast cyclic voltammetry (FCV) at carbon fibre microelectrodes was used to monitor endogenous noradrenaline (NA) efflux in superfused slices of bed nucleus of stria terminalis pars ventralis (BSTV) in 'real time'. NA efflux was evoked by local electrical stimulation at bipolar tungsten stimulating electrodes, Confirmation of the identity of the released species as NA was made on the basis of anatomical, electrochemical and pharmacological proofs. Firstly, the signal matched the NA innervation density: efflux of monoamine was greater in BSTV than in the pars dorsalis of the nucleus. Secondly, the voltammogram of the released species was indistinguishable from those of the catecholamines NA and dopamine (DA) but dissimilar to that of the indoleamine serotonin (5-hydroxytryptamine, 5-HT). Thirdly, amine efflux was influenced in a predictable fashion by the drugs tested. Tetrodotoxin (10(-6) M) or omission of Ca2+ from the superfusate reversibly reduced amine efflux by 90.2 and 88.0% respectively. Ro 4-1284 (10(-6) M) decreased amine efflux by 75.8%. Desipramine (5 x 10(-8) M), the selective NA uptake blocker, significantly increased amine efflux and uptake half-life (to 214.3 and 389.5% of control respectively). Fluvoxamine (5 x 10(-7) M) and GBR 12909 (3 x 10(-7) M), blockers of 5-HT and DA uptake respectively, had no effect on amine efflux, although fluvoxamine caused a modest (91.0%) increase in the uptake half-life. Pargyline (2 x 10(-6) M) affected neither efflux nor uptake. The combined anatomical, electrochemical and pharmacological data confirm that the monoamine detected in BSTV by local electrical stimulation was NA. Stimulated NA efflux was stable and reproducible over at least 2.5 h (longest period tested). This study demonstrates the ability of FCV to selectively monitor endogenous NA efflux and uptake in 'real time' and with high spatial resolution.