Migration of neural precursor cells derived from olfactory bulb in cochlear nucleus exposed to an augmented acoustic environment
Migration of neural precursor cells derived from olfactory bulb in cochlear nucleus exposed to an augmented acoustic environment
复制标题
暴露于增强声学环境的耳蜗核中嗅球衍生的神经前体细胞的迁移。
DOI：
10.1016/j.heares.2006.11.014
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发表时间：
2007-06-01
期刊：
影响因子：
2.8
通讯作者：
Liu, Shunli
中科院分区：
文献类型：
--
作者：
Chen, Yang;Qiu, Jianhua;Liu, Shunli
听觉神经系统的再生仍然是听力恢复的一个挑战。声信号可以诱导听觉系统中的位点特异性细胞替换。这一假设进行了测试与移植植入的神经前体细胞（NPC）沿着耳蜗核在成人主机，随后增强声刺激。从胚胎14-16天的嗅球中分离NPC，移植到耳蜗核内侧缘。标记的细胞存活至少2周，通过Hoechst 33342荧光和神经元标记物的免疫染色进行验证。在某些情况下，NPC定向迁移到听神经根。该观察结果表明，植入后，在增强的声学环境中，NPC从嗅球（013）沿着成人听神经存活和迁移。(c)2006年由Elsevier B. V.出版
The regeneration of the auditory neural system remains a challenge in hearing restoration. Acoustic signals may induce a site-specific cell replacement in the auditory system. This hypothesis was tested with grafted implantation of neural precursor cells (NPCs) along the cochlear nucleus in the adult host followed by an augmented acoustic stimulation. NPCs were obtained from the olfactory bulbs at embryonic day 14-16 and were transplanted into the inside border of cochlear nucleus. The labeled cells survived at least 2 weeks, verified by Hoechst 33342 fluorescence, and by immunostaining for a neuronal marker. In some cases NPCs had migrated directionally to the root of the auditory nerve. This observation demonstrates the survival and migration of NPCs from the olfactory bulb (013) along the adult auditory nerve in an augmented acoustic environment following implantation. (c) 2006 Published by Elsevier B.V.