Differential effects of a luteinizing-hormone-releasing hormone (LHRH) antagonist analogue on lordosis behavior induced by LHRH and the LHRH fragment Ac-LHRH5-10.
Differential effects of a luteinizing-hormone-releasing hormone (LHRH) antagonist analogue on lordosis behavior induced by LHRH and the LHRH fragment Ac-LHRH5-10.
复制标题
黄体生成素释放激素 (LHRH) 拮抗剂类似物对 LHRH 和 LHRH 片段 Ac-LHRH5-10 诱导的脊柱前凸行为的不同影响。
DOI：
10.1159/000125564
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发表时间：
1990
影响因子：
4.1
通讯作者：
Dudley,CA
中科院分区：
文献类型：
--
作者：
Moss,RL;Dudley,CA
促黄体生成激素释放激素（LHRH）十肽和由该十肽的最后6个氨基酸组成的LHRH片段Ac-LHRH 5 -10均已显示出增强切除卵巢的雌二醇-苯甲酸酯处理的雌性大鼠的脊柱前凸行为。虽然这两种肽的行为功效相似，但几项研究表明片段和十肽可能通过不同的机制起作用。本研究试图通过在输注Ac-LHRH 5 - 10或LHRH之前给予长效LHRH拮抗剂类似物来区分2种肽的行为作用。在没有拮抗剂给药的情况下，通过位于下丘脑腹内侧核（VMH）中的插管进行双侧输注后90分钟，十肽和片段在提升脊柱前凸姿势方面同样有效。将一种有效的LHRH拮抗剂类似物[Ac-LHRH，Pro 1，pCl，D-Phe 2，DTrp 3，6]-LHRH输注到VMH中，显著降低了随后用LHRH十肽处理的动物的脊柱前凸-坐骑比，但当动物随后输注Ac-LHRH 5 - 10片段时，对脊柱前凸行为没有显著影响。结果表明，LHRH增强交配行为是一个受体介导的事件，并表明Ac-LHRH 5 - 10片段通过一种与LHRH十肽不同的机制增强脊柱前凸行为。
Both the luteinizing-hormone-releasing hormone (LHRH) decapeptide and an LHRH fragment consisting of the last 6 amino acids of the decapeptide, Ac-LHRH5–10, have been shown to enhance lordosis behavior in ovariectomized, estradiol-benzoate treated female rats. Although the behavioral efficacy of the 2 peptides is similar, several lines of research suggest that the fragment and the decapeptide may act via different mechanisms. The present study attempted to differentiate the behavioral action of the 2 peptides by administering a long-acting LHRH antagonist analogue prior to infusion of either Ac-LHRH5–10or LHRH. Without antagonist administration, the decapeptide and the fragment were equally effective in elevating lordotic posturing 90 min after bilateral infusion through cannulae positioned in the ventromedial nucleus of the hypothalamus (VMH). Infusion of a potent LHRH antagonist analogue [Ac-dehydro, Pro1, pCl, D-Phe2, DTrp3,6]-LHRH, into the VMH significantly reduced the lordosis-to-mount ratio in animals subsequently treated with the LHRH decapeptide, but had no significant effect on lordotic behavior when the animals were subsequently infused with the Ac-LHRH5–10fragment. The results indicate that LHRH enhancement of mating behavior is a receptor-mediated event and suggest that the Ac-LHRH5–10fragment enhances lordotic behavior via a mechanism that is distinct from that of the LHRH decapeptide.