A novel role for histone methyltransferase KYP/SUVH4 in the control of Arabidopsis primary seed dormancy.
A novel role for histone methyltransferase KYP/SUVH4 in the control of Arabidopsis primary seed dormancy.
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DOI：
10.1111/j.1469-8137.2011.03969.x
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发表时间：
2012-02
期刊：
影响因子：
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通讯作者：
Jian Zheng;Fengying Chen;Zhi Wang;Hong Cao;Xiaoying Li;X. Deng;W. Soppe;Yong Li;Yong-xiu Liu
中科院分区：
文献类型：
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作者：
Jian Zheng;Fengying Chen;Zhi Wang;Hong Cao;Xiaoying Li;X. Deng;W. Soppe;Yong Li;Yong-xiu Liu
·种子休眠控制发芽，在植物的生命周期中起着至关重要的作用。染色质修饰参与了种子休眠的调控，但其内在机制却知之甚少。·组蛋白H3赖氨酸9二甲基化需要KYP/SUV H4。该基因的突变会导致种子休眠增加。KYP/SUVH 4过表达的拟南芥植物显示休眠减少。KYP/SUVH 4的表达受脱落酸（阿坝）和赤霉素（GA）的调节。kyp-2和suvh 4 -2/suvh 5突变体的种子萌发对ABA和多效唑的敏感性略有增强，而KYP/SUVH 4过表达的种子萌发对ABA和多效唑的敏感性减弱。在kyp-2突变体中，包括DOG 1和ABI 3在内的几个休眠相关基因显示出表达水平增加，这与KYP/SUVH 4在基因转录中的负作用一致。遗传分析表明，DOG 1和HUB 1对KYP/SUVH 4具有上位性，表明这些基因通过相同的遗传途径调节种子休眠。
• Seed dormancy controls germination and plays a crucial role in the life cycle of plants. Chromatin modifications are involved in the regulation of seed dormancy; however, little is known about the underlying mechanism. • KYP/SUVH4 is required for histone H3 lysine 9 dimethylation. Mutations in this gene cause increased seed dormancy. KYP/SUVH4-overexpressing Arabidopsis plants show decreased dormancy. KYP/SUVH4 expression is regulated by abscisic acid (ABA) and gibberellins (GA). The sensitivity of seed germination to ABA and paclobutrazol (PAC) is enhanced slightly in kryptonite-2 (kyp-2) and suvh4-2/suvh5 mutants, but weakened in KYP/SUVH4-overexpressing plants. • In the kyp-2 mutant, several dormancy-related genes, including DOG1 and ABI3, show increased expression levels, in agreement with a negative role for KYP/SUVH4 in gene transcription. • Genetic analysis showed that DOG1 and HUB1 are epistatic to KYP/SUVH4, suggesting that these genes regulate seed dormancy in the same genetic pathway.