Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice (Oryza sativa) plants
Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice (Oryza sativa) plants
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DOI：
10.1111/j.1365-313x.2010.04146.x
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发表时间：
2010-04-01
期刊：
影响因子：
7.2
通讯作者：
Chen, Shou-Yi
中科院分区：
文献类型：
--
作者：
Ouyang, Shou-Qiang;Liu, Yun-Feng;Chen, Shou-Yi
P>；受体样激酶(RLKs)在植物的生长发育和对环境胁迫的响应中起着重要的作用。克隆了水稻富含亮氨酸重复序列的RLK基因OsSIK1，并对其进行了鉴定。在有Mn2+存在的情况下，OsSIK1显示出激酶活性，并且OsSIK1的激动域具有自我磷酸化和磷酸化髓鞘碱性蛋白(MBP)的能力。OsSIK1启动子-GUS分析表明，OsSIK1主要在水稻的茎和小穗中表达。OsSIK1的表达主要受盐、干旱和过氧化氢处理的诱导。转OsSIK1基因水稻植株表现出比对照植株更强的耐盐性和抗旱性。相反，基因敲除突变体SIK1-1和SIK1-2以及RNA干扰(RNAi)植物对干旱和盐胁迫都很敏感。在OsSIK1过表达的植株中，过氧化物酶、超氧化物歧化酶和过氧化氢酶的活性显著提高。此外，3，3‘-二氨基联苯胺(DAB)染色显示，过表达OsSIK1的植株叶片中过氧化氢的积累量远低于突变体、RNAi植株和对照植株。我们还发现OsSIK1影响水稻远轴和近轴叶表皮的气孔密度。这些结果表明，OsSIK1通过激活抗氧化系统在水稻耐盐性和抗旱性中发挥重要作用。
P>Receptor-like kinases (RLKs) play essential roles in plant growth, development and responses to environmental stresses. A putative RLK gene, OsSIK1, with extracellular leucine-rich repeats was cloned and characterized in rice (Oryza sativa). OsSIK1 exhibits kinase activity in the presence of Mn2+, and the OsSIK1 kinase domain has the ability to autophosphorylate and phosphorylate myelin basic protein (MBP). OsSIK1 promoter-GUS analysis revealed that OsSIK1 is expressed mainly in the stem and spikelet in rice. The expression of OsSIK1 is mainly induced by salt, drought and H2O2 treatments. Transgenic rice plants with overexpression of OsSIK1 show higher tolerance to salt and drought stresses than control plants. On the contrary, the knock-out mutants sik1-1 and sik1-2, as well as RNA interference (RNAi) plants, are sensitive to drought and salt stresses. The activities of peroxidase, superoxide dismutase and catalase are enhanced significantly in OsSIK1-overexpressing plants. Also, the accumulation of H2O2 in leaves of OsSIK1-overexpressing plants is much less than that of the mutants, RNAi plants and control plants, as measured by 3,3'-diamino benzidine (DAB) staining. We also show that OsSIK1 affects stomatal density in the abaxial and adaxial leaf epidermis of rice. These results indicate that OsSIK1 plays important roles in salt and drought stress tolerance in rice, through the activation of the antioxidative system.