Kinematics of intracellular chlamydiae provide evidence for contact-dependent development.
Kinematics of intracellular chlamydiae provide evidence for contact-dependent development.
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细胞内衣原体的运动学为接触依赖性发育提供了证据。
DOI：
10.1128/jb.00293-09
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发表时间：
2009
影响因子：
3.2
通讯作者：
Bavoil,PatrikM
中科院分区：
文献类型：
--
作者：
Wilson,DavidP;Whittum-Hudson,JudithA;Timms,Peter;Bavoil,PatrikM
衣原体发育的一个关键过程是由复制网状体（RB）分化为感染性初等体（EB）。我们提出的实验证据，以提供支持的接触依赖的假设，以解释涉及分化的触发。我们记录了沙眼衣原体感染的McCoy细胞在发育过程中的关键时刻的实时成像，并追踪了单个衣原体颗粒的时空轨迹。我们发现粒子的运动与发育有关。在发育的早期到中期，RBs有轻微的摆动。在感染后24小时（估计RB向EB分化开始后），颗粒的平均速度急剧增加。我们还研究了一个含有EBs、RBs和异常增大的应激衣原体的青霉素补充培养。接近不动的增大颗粒与它们继续附着在衣原体包合膜（CIM）上一致。我们发现速度和颗粒大小/类型之间存在显著的负非线性关联，这进一步支持了颗粒在RB向EB分化开始时变得不受束缚的假设。本研究建立了衣原体的运动特性与发育阶段之间的关系，其中摆动的RB逐渐与CIM失去联系，RB脱离CIM与后期分化的开始是一致的。
A crucial process of chlamydial development involves differentiation of the replicative reticulate body (RB) into the infectious elementary body (EB). We present experimental evidence to provide support for a contact-dependent hypothesis for explaining the trigger involved in differentiation. We recorded live-imaging ofChlamydia trachomatis-infected McCoy cells at key times during development and tracked the temporospatial trajectories of individual chlamydial particles. We found that movement of the particles is related to development. Early to mid-developmental stages involved slight wobbling of RBs. The average speed of particles increased sharply at 24 h postinfection (after the estimated onset of RB to EB differentiation). We also investigated a penicillin-supplemented culture containing EBs, RBs, and aberrantly enlarged, stressed chlamydiae. Near-immobile enlarged particles are consistent with their continued tethering to the chlamydial inclusion membrane (CIM). We found a significantly negative, nonlinear association between speed and size/type of particles, providing further support for the hypothesis that particles become untethered near the onset of RB to EB differentiation. This study establishes the relationship between the motion properties of the chlamydiae and developmental stages, whereby wobbling RBs gradually lose contact with the CIM, and RB detachment from the CIM is coincidental with the onset of late differentiation.