Reston virus in domestic pigs in China
Reston virus in domestic pigs in China
复制标题
中国家猪中的雷斯顿病毒
DOI：
10.1007/s00705-012-1477-6
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发表时间：
2014-05-01
影响因子：
2.7
通讯作者：
Zeng, Qiaoying
中科院分区：
文献类型：
--
作者：
Pan, Yangyang;Zhang, Wen;Zeng, Qiaoying
历史上，莱斯顿病毒（RESTV）已被发现与疾病的爆发，只有在非人类灵长类动物。2008年首次报告了家猪的传播。本研究报告了中国家猪中莱斯顿病毒（RESTV）的发现、分子检测和系统发育分析。2011年2 ~ 9月，从上海市3个猪场采集了137份猪繁殖与呼吸综合征（PRRS）典型临床症状死亡的脾脏标本，经RT-PCR检测证实为猪繁殖与呼吸综合征病毒（PRRSV）感染。其中RESTV阳性率为2.92%（4/137）。所有阳性仔猪均为8周龄以下的混合感染猪繁殖与呼吸综合征病毒的仔猪。发现序列与先前在来自菲律宾的家猪和食蟹猴中发现的两种RESTV变体的序列具有96.1%-98.9%的序列相似性。因此，我们得出结论，RESTV存在于中国上海的家猪中。
Historically, Reston virus (RESTV) has been found to be associated with outbreaks of disease only in nonhuman primates. Its spread to domestic pigs was reported for the first time in 2008. In this study, we report the discovery, molecular detection, and phylogenetic analysis of Reston virus (RESTV) in domestic pigs in China. A total of 137 spleen specimens from pigs that died after showing typical clinical signs of porcine reproductive and respiratory syndrome (PRRS), and for which infection with porcine reproductive and respiratory syndrome virus (PRRSV) was confirmed by RT-PCR, were collected from three farms in Shanghai from February to September 2011. Of these samples, 2.92 % (4/137) were found to be positive for RESTV. All of the positive piglets were under the age of 8 weeks and were co-infected with PRRSV. Sequences were found that shared 96.1 %-98.9 % sequence similarity with those of two RESTV variants that had been discovered previously in domestic pigs and cynomolgus macaques from the Philippines. We therefore conclude that RESTV was present in domestic pigs in Shanghai, China.