Methodology for Reconstructing Early Zebrafish Development From In Vivo Multiphoton Microscopy
Methodology for Reconstructing Early Zebrafish Development From In Vivo Multiphoton Microscopy
复制标题
从体内多光子显微镜重建早期斑马鱼发育的方法
DOI：
10.1109/tip.2011.2177911
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发表时间：
2012
影响因子：
10.6
通讯作者：
Andrés Santos
中科院分区：
文献类型：
--
作者：
M. Luengo;J. L. Rubio;E. Faure;T. Savy;L. Duloquin;N. Olivier;D. Pastor;M. Ledesma;D. Débarre;P. Bourgine;E. Beaurepaire;N. Peyriéras;Andrés Santos
研究早期斑马鱼胚胎发生过程中的细胞动力学需要特定的图像采集和分析策略。多谐波显微镜，即第二和第三谐波世代，可以对未染色斑马鱼胚胎从 1 到 1000 细胞阶段的细胞分裂和细胞膜进行成像。本文介绍了专用图像处理管道（跟踪和分割）的设计和实现，用于重建这些发育阶段的细胞动力学。该方法允许重建细胞谱系树，包括分裂时间、空间坐标和细胞形状，直到 1000 个细胞阶段，具有微小的时间精度和微米的空间分辨率。数字胚胎的数据分析提供了早期斑马鱼胚胎发生的广泛定量描述。
Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebrafish embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebrafish embryogenesis.
影响因子：
4.6
作者：
D. Kane;C. Kimmel
通讯作者：
D. Kane;C. Kimmel