Genome-wide gene expression in an Arabidopsis cell suspension
Genome-wide gene expression in an Arabidopsis cell suspension
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DOI：
10.1023/b:plan.0000019059.56489.ca
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发表时间：
2003-11-01
影响因子：
5.1
通讯作者：
Murray, JAH
中科院分区：
文献类型：
--
作者：
Menges, M;Hennig, L;Murray, JAH
植物细胞悬浮培养是研究细胞过程的宝贵模型。在这里，我们开发了最近描述的拟南芥悬浮培养物MM2d作为转录谱分析平台，通过Affyphysis ATH1微阵列。在正常培养生长期间，在同步细胞周期重新进入期间和在同步细胞周期进程期间的基因表达谱的分析提供了在高等植物系统中的基因表达响应的独特的综合视图。特别引人注目的是，超过14 000个属于所有定义的类别的基因的表达可以被可靠地检测到，这表明来自文化的转录谱的数据集的综合和比较分析是一个强大的方法来确定参与广泛的生物过程的候选成分。独立的细胞周期同步性方法的组合分析允许识别明显受细胞周期调节但最有可能响应于同步性诱导的基因。因此，我们提出了一个完整的全基因组的观点，植物悬浮培养的转录谱，并确定了一套完善的1082个细胞周期调控基因，在很大程度上独立于同步方法。
Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platformby means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during synchronous cell cycle re-entry and during synchronous cell cycle progression provides a unique integrated view of gene expression responses in a higher-plant system. Particularly striking is that expression of over 14 000 genes belonging to all defined categories can be reliably detected, suggesting that integrated and comparative analysis of data sets derived from transcript profiling of cultures is a powerful approach to identify candidate components involved in a wide range of biological processes. Combinatorial analysis of independent cell cycle synchrony methods allows the identification of genes that are apparently cell-cycle-regulated but are most likely responding to the induction of synchrony. We thus present an integrated genome-wide view of the transcriptional profile of a plant suspension culture and identify a refined set of 1082 cell cycle regulated genes largely independent of synchrony method.