New insight into protein-nanomaterial interactions with UV-visible spectroscopy and chemometrics: human serum albumin and silver nanoparticles.
New insight into protein-nanomaterial interactions with UV-visible spectroscopy and chemometrics: human serum albumin and silver nanoparticles.
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DOI：
10.1039/c3an01818k
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
Yong Wang;Y. Ni
中科院分区：
文献类型：
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作者：
Yong Wang;Y. Ni
近年来，蛋白质纳米复合物在生物分析科学、生物传感器、生物催化、生物燃料电池和生物基纳米器件等领域有着广泛的应用前景，因此人们对蛋白质纳米复合物的研究和制备进行了大量的工作。我们理解蛋白质纳米复合物的一个重要方面是定量地了解蛋白质如何与纳米材料相互作用。在这份报告中，人血清白蛋白（HSA）和柠檬酸盐包被的银纳米粒子（AgNPs）被选为蛋白质-纳米材料相互作用的案例研究。紫外-可见光谱与交替最小二乘法（MCR-ALS）算法的多元曲线分辨率的AgNPs-HSA相互作用的详细研究首次被利用。化学计量学工具的引入允许提取两种主要吸收纯物质（AgNPs和AgNPs-HSA缀合物）的动力学曲线、光谱和分布图。这些解决的配置文件，然后进行分析，以给出的热力学，动力学和结构信息的HSA结合AgNPs。采用透射电镜、圆二色光谱和傅里叶变换红外光谱对复合体系进行了表征。此外，利用MCR-ALS分辨的吸收纯物种浓度，制备了灵敏的HSA光谱生物传感器。结果表明，HSA纳米传感器的线性范围为1.9 nM ~ 45.0 nM，检测限为0.9 nM。据信，所提出的方法将发挥重要作用，在制造和优化的一个强大的纳米生物传感器或交叉反应传感器阵列的检测和识别的生物成分。
In recent years, great efforts have focused on the exploration and fabrication of protein nanoconjugates due to potential applications in many fields including bioanalytical science, biosensors, biocatalysis, biofuel cells and bio-based nanodevices. An important aspect of our understanding of protein nanoconjugates is to quantitatively understand how proteins interact with nanomaterials. In this report, human serum albumin (HSA) and citrate-coated silver nanoparticles (AgNPs) are selected as a case study of protein-nanomaterial interactions. UV-visible spectroscopy together with multivariate curve resolution by alternating least squares (MCR-ALS) algorithm is first exploited for the detailed study of AgNPs-HSA interactions. Introduction of the chemometrics tool allows extracting the kinetic profiles, spectra and distribution diagrams of two major absorbing pure species (AgNPs and AgNPs-HSA conjugate). These resolved profiles are then analysed to give the thermodynamic, kinetic and structural information of HSA binding to AgNPs. Transmission electron microscopy, circular dichroism spectroscopy and Fourier transform infrared spectroscopy are used to further characterize the complex system. Moreover, a sensitive spectroscopic biosensor for HSA is fabricated with the MCR-ALS resolved concentration of absorbing pure species. It is found that the linear range for the HSA nanosensor was from 1.9 nM to 45.0 nM with a detection limit of 0.9 nM. It is believed that the proposed method will play an important role in the fabrication and optimization of a robust nanobiosensor or cross-reactive sensors array for the detection and identification of biocomponents.