The silver reduction method for the demonstration of connective-tissue fibres.
The silver reduction method for the demonstration of connective-tissue fibres.
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DOI：
10.1002/path.1700240109
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发表时间：
1921-01-01
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影响因子：
--
通讯作者：
Perdrau, JR
中科院分区：
文献类型：
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作者：
Perdrau, JR
在Bielschowsky染色轴圆柱体等的方法中，在冷冻切片中，结缔组织纤维偶尔被染成深黑色。如果在将切片放入银预浴之前，用Weigert髓鞘染色法对切片进行脱色处理，则可以获得这些纤维的清晰染色。这种方法最适合在冷冻切片机上切下的薄片。明胶、石蜡或火棉胶包埋不是禁忌症。细胞核、轴柱、神经原纤维等，这种方法对于一般的日常工作来说太长了，但当需要比苯胺染料染色更持久的准备时，这种方法应该是有价值的。最小的纤维被染成深黑色，在用明矾胭脂红复染后再用苦味酸复染的切片中非常突出。苏木精和伊红可用作复染剂，但对比度不太好。程序应如下：-非常薄的切片用25%的锅处理。用高锰酸盐浸泡5分钟或更长时间，洗涤并置于Doppler脱色器中直至完全白色。然后将它们在几次更换的蒸馏水中彻底洗涤过夜，最后按照Da Fano对Bielschowsky方法的修改进行染色（1）。最好将切片在氨化银浴中放置40至60分钟，而不是如轴圆柱体等所述的20分钟。切片在锅中放置的时间越长。高锰酸盐浴后，随后的核复染将不那么锐利。在调色和固定后，
IN Bielschowsky's method of staining axis cylinders, etc., in frozen sections connective-tissue fibres are occasionally stained an intense black. A sharp stain for these fibres can be obtained if the sections are treated by Pal's decolorising method for Weigert's myelin stain before the sections are put in the preliminary bath of silver nitrate. The method works best with thin sections cut on the freezing micro-tome. Embedding in gelatin, paraffin, or celloidin is not a contraindication. Nuclei, axis cylinders, neurofibrils, etc., are all left absolutely unstained.The method is too long for ordinary routine work, but should be valuable when more permanent preparations are wanted than those stained with the aniline dyes. The smallest fibrils are stained an intense black, and stand out well in sections counterstained with alum carmine followed by picric acid. Hæmatoxylin and eosin can be used as counterstains, but the contrast is not so good. The procedure should be as follows:-Very thin sections are treated with 25 per cent. pot. permanganate for five minutes or longer, washed and placed in Pal's decoloriser until quite white. They are then thoroughly washed in several changes of distilled water overnight, and finally stained as in Da Fano's modification of Bielschowsky's method (1). It is best to leave the sections forty to sixty minutes in the ammoniated silver bath, instead of twenty minutes as described for axis cylinders, etc. The longer the sections are left in the pot. permanganate bath the less sharp will be the subsequent nuclear counterstain. After toning and fixing, the sections