Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan
Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan
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DOI：
10.1016/j.vetpar.2010.11.008
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发表时间：
2011-03-10
影响因子：
2.6
通讯作者：
Sasai, Kazumi
中科院分区：
文献类型：
--
作者：
Matsubayashi, Makoto;Carreno, Ramon A.;Sasai, Kazumi
囊等孢菌从狗、猫和浣熊狗的粪便中分离了Cystoisospora，在小亚基核糖体RNA基因位点处进行了测序，并与其他囊等孢菌属物种进行了比较。犬和浣熊体内的囊等孢子虫卵囊与C. Ohioensis和cat分离物与C.猫犬、浣熊犬和猫的序列与C. ohioensis和C.，分别。DNA序列的系统发育分析表明，狗和浣熊狗分离株与其他囊等孢菌属的物种嵌套在一个分支中。包括C. ohioensis、C. belli，和C.奥尔洛维。猫分离物与C.猫是从狗和浣熊狗群中分离出来的一个分支。我们报告了这些囊等孢菌序列中的序列变异，并确定浣熊狗是囊等孢菌的另一种食肉动物宿主。感染狗(C)2010 Elsevier B.V.保留所有权利。
Cystoisospora spp. from feces in dogs, cats, and raccoon dogs were isolated, sequenced at the small subunit ribosomal RNA gene locus and compared to other Cystoisospora spp. Cystoisospora oocysts from dogs and raccoon dogs were morphologically similar with those of C. ohioensis, and cat isolates were similar with those of C. felis. The sequences from dogs and raccoon dogs, and cats have a homology with C. ohioensis and C. fells, respectively. Phylogenetic analysis of the DNA sequences showed that the dog and raccoon dog isolates were nested in a clade with other Cystoisospora spp. including C. ohioensis, C. belli, and C. orlovi. The cat isolate formed a sister group with C. felis that was a separate clade from the dog and raccoon dog group. We report sequence variation in these Cystoisospora sequences and have identified raccoon dogs as another carnivore host for Cystoisospora spp. infecting dogs. (C) 2010 Elsevier B.V. All rights reserved.