The role of photorespiration during astaxanthin accumulation in Haematococcus pluvialis (Chlorophyceae).
The role of photorespiration during astaxanthin accumulation in Haematococcus pluvialis (Chlorophyceae).
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DOI：
10.1016/j.plaphy.2016.05.029
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发表时间：
2016-10
期刊：
影响因子：
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通讯作者：
Chunhui Zhang;Litao Zhang;Jianguo Liu
中科院分区：
文献类型：
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作者：
Chunhui Zhang;Litao Zhang;Jianguo Liu
以往对雨生红球藻的研究主要集中在生长和虾青素积累方面。然而，光呼吸和虾青素积累之间的关系尚未阐明。本研究旨在探讨光呼吸在雨生红球藻积累虾青素过程中的作用。在虾青素积累过程中，当光呼吸被其特异性抑制剂羧甲氧胺抑制时，虾青素含量显著降低。抑制光呼吸并不改变培养过程中的干重、叶绿素含量和OJIP瞬变;但抑制光呼吸显著降低了光系统II的光化学和总光合放氧能力。此外，光呼吸的限制与虾青素积累的减少是同步的。这些结果表明，雨生红球藻的光呼吸途径可以促进虾青素的积累。推测光呼吸通过以下途径促进雨生红球藻对虾青素的积累：（1）光呼吸直接影响雨生红球藻对3-磷酸甘油酸（PGA）的积累，而PGA的积累与虾青素的积累密切相关;（2）光呼吸途径通过影响光合作用的暗反应间接影响PGA的积累，从而促进雨生红球藻对虾青素的积累。
Most previous studies on Haematococcus pluvialis have been focused on growth and astaxanthin accumulation. However, the relationships between photorespiration and astaxanthin accumulation have not been clarified. The purpose of this study was to examine the role of photorespiration during the process of astaxanthin accumulation inH. pluvialis. During astaxanthin accumulation, the astaxanthin content was reduced significantly when photorespiration was inhibited by its specific inhibitor, carboxymethoxylamine. The inhibition of photorespiration did not change the dry weight, chlorophyll content and OJIP transients during the incubation; however, the inhibition of photorespiration significantly decreased the photochemistry of photosystem II and total photosynthetic O2evolution capacity. Moreover, the restriction in photorespiration was synchronized with a decrease of astaxanthin accumulation. These results suggest that the photorespiratory pathway inH. pluvialiscan accelerate astaxanthin accumulation. We speculate that photorespiration can enhance astaxanthin accumulation in the following ways: (i) photorespiration directly affects the glycerate-3-phosphate (PGA) level, which is intrinsically related to the accumulation of astaxanthin inH. pluvialis; (ii) the photorespiratory pathway indirectly affects the PGA level by effecting the dark reactions of photosynthesis, which then results in the enhancement of astaxanthin accumulation inH. pluvialis.