Preparation of porous PLGA/HA/collagen scaffolds with supercritical CO2 and application in osteoblast cell culture
Preparation of porous PLGA/HA/collagen scaffolds with supercritical CO2 and application in osteoblast cell culture
复制标题
超临界CO2制备多孔PLGA/HA/胶原支架及其在成骨细胞培养中的应用
DOI：
10.1016/j.supflu.2011.07.003
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发表时间：
2011-10-01
影响因子：
3.9
通讯作者：
Zhang, Zhi-Qi
中科院分区：
文献类型：
--
作者：
Mou, Zhao-Li;Zhao, Li-Jun;Zhang, Zhi-Qi
用于细胞培养的生物兼容三维支架可能会促进修复受损人体组织的方法。采用超临界CO2饱和技术制备了一种新型的聚乳酸-乙醇酸-羟基磷灰石-胶原杂化多孔支架。研究了超临界CO2处理后不同组成支架的膨胀系数，以选择适合三维培养的最佳组成。改变超临界CO2工艺条件，如饱和温度、饱和时间和饱和压力，评价它们对孔隙结构的影响。结果表明，通过控制这些工艺条件，可以控制支架的孔径和孔隙率。用环境扫描电子显微镜、能量色散X射线光谱仪、傅立叶变换红外光谱和X射线衍射仪对样品进行了表征。终于来了。通过电子显微镜和共聚焦显微镜检测，MG-63细胞成功地在多孔支架上培养，证实了这种新型复合多孔支架的生物相容性。(C)2011爱思唯尔B.V.保留所有权利。
Biocompatible three-dimensional scaffolds for cell culturing may facilitate methods for the repair of damaged human tissues. A novel hybrid porous scaffold of poly(lactic-co-glycolic acid), hydroxyapatite and collagen was prepared using a supercritical CO2 saturation technique. Expansion factors of scaffolds with different compositions were studied after supercritical CO2 treatment to choose the optimal composition for three-dimensional culture. The supercritical CO2 process conditions, such as saturation temperature, saturation time and saturation pressure were varied to evaluate their influence on pore structure. The results showed that the pore size and porosity of the scaffold could be controlled by manipulating these process conditions. The porous samples were characterized by environmental scanning electron microscopy, energy-dispersive X-ray spectroscope, Fourier transform infrared spectroscopy and X-ray diffractometry. Finally. MG-63 cells were successfully cultured on the porous scaffold as assessed by electron and confocal microscopy, confirming the biocompatibility of this new hybrid porous scaffold. (C) 2011 Elsevier B.V. All rights reserved.