Rapid alkalinization factor (RALF) genes are related to genic male sterility in Chinese cabbage (Brassica rapa L. ssp pekinensis)
Rapid alkalinization factor (RALF) genes are related to genic male sterility in Chinese cabbage (Brassica rapa L. ssp pekinensis)
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快速碱化因子（RALF）基因与大白菜（Brassica rapa L. ssp pekinensis）的雄性不育相关
DOI：
10.1016/j.scienta.2017.07.041
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发表时间：
2017
影响因子：
4.3
通讯作者：
Feng Hui
中科院分区：
文献类型：
--
作者：
Shi Fengyan;Zhou Xue;Liu Zhiyong;Feng Hui
雄性不育是芸苔属植物杂交制种的一种普遍而广泛的方法。ssp.pekinensis (B。以及一些与花药和花粉发育有关的基因可能在花粉育性中起重要作用。因此，在本研究中，我们进行了全基因组扫描，并鉴定了b中theRALFgene家族的38个基因。根据系统发育树将其分为4个支系。其中36个序列只包含外显子；另外两个包含外显子和内含子序列。确定了38个ralf基因的保守基序。重复的dralfgene对的Ka/Ks比小于1，表明它们经过了纯化选择。此外，通过RNA-Seq在AB系‘AB01’的可育雄芽和不育雄芽之间共鉴定出14个ralf样差异表达基因（deg），这些基因均在AB系‘AB01’的可育雄芽中高表达或特异性表达。qRT-PCR分析证实，这14个RALF基因在AB系‘AB01’中的表达模式与其他3个基因雄性不育（GMS） AB系‘AB02’、‘AB03’和‘AB04’中的表达模式相似，表明theRALFgenes可能与b的花粉发育有关。拉伯。同时，qRT-PCR结果验证了RNA-Seq转录组的准确性和可靠性。该研究将为探索b的GMS AB系不育的分子机制提供有价值的信息。拉伯。
Male sterility is commonly and widely used for hybrid seed production inBrassica rapaL. ssp.pekinensis(B. rapa), and some genes related to anther and pollen development may play significant roles in pollen fertility. Hence, in this study, we performed whole-genome scanning and identified 38 genes from theRALFgene family inB. rapa, which were grouped into four clades according to the phylogenetic tree. Thirty-six of the sequences contained only exons; the other two contained both exonic and intronic sequences. Conserved motifs of the 38RALFgenes were identified. The Ka/Ks ratios of duplicatedRALFgene pairs were less than 1, indicating that they had gone through purifying selection. Moreover, a total of 14RALF-like differentially expressed genes (DEGs) were identified by RNA-Seq between male fertile and male sterile buds, and all were highly or specifically expressed in male fertile buds of the AB line ‘AB01’. A qRT-PCR analysis confirmed that the expression patterns of the 14 RALF DEGs in the AB line ‘AB01’ were similar to those in the other three genic male sterility (GMS) AB lines ‘AB02’, ‘AB03’, and ‘AB04’, indicating that theRALFgenes may be related to pollen development inB. rapa. Meanwhile, the results of qRT-PCR validated the RNA-Seq transcriptome as accurate and reliable. This study will provide valuable information for exploring the molecular mechanism underlying sterility in GMS AB lines ofB. rapa.