Laccase biosensor fabricated on flower-shaped yolk-shell SiO2 nanospheres for catechol detection
Laccase biosensor fabricated on flower-shaped yolk-shell SiO2 nanospheres for catechol detection
复制标题
在花形蛋黄壳二氧化硅纳米球上制备漆酶生物传感器用于儿茶酚检测
DOI：
10.1016/j.colsurfa.2017.10.086
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zhou Guowei
中科院分区：
文献类型：
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作者：
Zheng Yujie;Wang D;an;Li Zhikai;Sun Xuefeng;Gao Tingting;Zhou Guowei
In this study, we developed a facile one–pot strategy for fabricating flower–shaped yolk–shell SiO2nanospheres (FYSSns) using cetyltrimethylammonium bromide–polyvinylpyrrolidone–cyclohexane–ethanol–water vesicle–microemulsion composite aggregation as templates through a simple solvothermal route. FYSSns displayed yolk–shell structures with flower-shaped yolks (260–320 nm) with ordered radial mesochannels (∼7 nm), large cavities (100–120 nm), and thin silica shells (20–30 nm). High laccase immobilization amounts were achieved using FYSSns as supporter, and the laccase–modified FYSSns were applied to the electrochemical sensor with high selectivity for detecting catechol. Under the optimized conditions, the proposed sensor showed a wide linear range from 12.5–450 μM over catechol concentration and a low detection limit of 1.6 μM. The outstanding performance was attributed to the advantages of FYSSns, such as large specific surface area and its own mesochannel structure.
