Ameliorative effects of Bacillus subtilis ANSB01G on zearalenone toxicosis in pre-pubertal female gilts
Ameliorative effects of Bacillus subtilis ANSB01G on zearalenone toxicosis in pre-pubertal female gilts
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DOI：
10.1080/19440049.2014.976845
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发表时间：
2015-04-03
影响因子：
2.9
通讯作者：
Ji, Cheng
中科院分区：
文献类型：
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作者：
Zhao, Lihong;Lei, Yuanpei;Ji, Cheng
本研究旨在研究玉米赤霉烯酮(ZEA)对青春期前母猪生长性能、生殖器官、血清激素和组织病理学的影响，并评价枯草芽孢杆菌ANSB01G对母猪ZEA中毒的缓解效果。将18头青春期前母猪随机分成三个处理，每个处理一个重复。分别饲喂3种日粮24天：对照组饲喂普通玉米基础日粮，处理1(T1)用自然污染的玉米代替基础日粮中所有的正常玉米(最终浓度为238.57亩玉米)，处理2(T2)由T1日粮和2kgT(-1)的ANSB01G发酵干培养物混合制成。结果表明，与对照组相比，日粮中添加ZEA显著增加了T1母猪的外阴大小和生殖器官重量，在自然污染ZEA的日粮中添加ANSB01G明显改善了这些症状，T2组也观察到了这一现象。T1组仔猪血清卵泡刺激素(FSH)、黄体生成素(LH)和雌二醇(E_2)水平无显著变化，但催乳素(PRL)水平显著升高。与对照组相比，T1组母猪子宫体部细胞明显增大，脂肪变性，肝细胞肿胀、炎症、淋巴细胞浸润。ANSB01G的存在可以缓解ZEA引起的这些高雌激素效应，保持母猪身体的正常和健康状态。提示在较短的时间内饲喂低剂量的玉米对母猪的生殖器官有严重的影响，添加枯草芽孢杆菌ANSB01G可有效缓解玉米中毒。
The purpose of this research was to investigate the toxicity of zearalenone (ZEA) on the growing performance, genital organs, serum hormones and histopathological changes of pre-pubertal female gilts, and to evaluate the efficacy of Bacillus subtilis ANSB01G in alleviating ZEA toxicosis in gilts. Eighteen pre-pubertal female gilts were randomly allocated to three treatments with one replicate per treatment. The gilts were fed following three diets for 24days: the Control group was given a basic diet with normal corn; Treatment 1 (T1) was prepared by substituting corn naturally contaminated with ZEA for all normal corn in the basic diet (with a final concentrations of 238.57 mu gkg(-1) of ZEA); and Treatment 2 (T2) was prepared by mixing the T1 diet with 2kgT(-1) of fermented-dried culture of ANSB01G. The results showed that the presence of ZEA in diets significantly increased the vulva size and reproductive organ weight of the T1 gilts as compared with the Control group, and the addition of ANSB01G to diet naturally contaminated with ZEA obviously ameliorated these symptoms, as was observed in the T2 group. The presence of low doses of ZEA in the T1 diet had no significant effect on the level of follicle-stimulating hormone (FSH), luteotrophic hormone (LH) or serum oestradiol (E2) in the serum of gilts, but the prolactin (PRL) level in group T1 increased significantly. The gilts of the T1 group exhibited conspicuous cell enlargement and fatty degeneration of the corpus uteri, swelling, inflammation and lymphocyte infiltration of liver cells as compared with the Control group. The presence of ANSB01G can alleviate these hyperoestrogenic effects caused by ZEA, maintaining the body of gilt in a normal and healthy status. It is suggested that reproductive organs of gilts are seriously affected even if they are fed a low dose of ZEA in less time, and the addition of B. subtilis ANSB01G can effectively alleviate ZEA toxicosis in gilts.