Exercise as a new physiological stimulus for brown adipose tissue activity
Exercise as a new physiological stimulus for brown adipose tissue activity
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DOI：
10.1016/j.numecd.2012.01.013
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发表时间：
2013-06-01
影响因子：
3.9
通讯作者：
Cuppini, R.
中科院分区：
文献类型：
--
作者：
De Matteis, R.;Lucertini, F.;Cuppini, R.
背景和目的：棕色脂肪组织（BAT）在机体能量消耗中起着重要作用，可对抗肥胖和肥胖相关疾病。BAT活动由交感神经系统（SNS）维持。由于SNS在体力活动中被大量激活，我们研究了耐力跑训练对年轻大鼠BAT的影响，以阐明运动训练对棕色细胞活性和募集状态的作用。方法和结果：雄性10周龄Sprague道利大鼠在运动跑步机上训练（约为VO（2）max的60%），每周5天，持续1周和6周。从形态学和分子生物学的角度对耐力训练的影响进行了研究，结果表明，跑步训练对BAT的形态、交感神经张力和血管化的影响与刺激的持续时间无关，但在功能上，BAT的产热作用微弱增加（UCP-1无差异），PGC-1 α表达增加，MCT-1膜定位增加，提示BAT具有一种新的功能。最后一次训练后48小时，内脏脂肪增加了FOXC 2的表达，并出现了一些UCP-1少房和多房脂肪细胞簇。结论：运动似乎对BAT产热的刺激作用较弱，但令人惊讶的是，没有所谓的代谢功能减退效应。观察到的内脏脂肪的布朗宁，通过假定的白色到棕色的转分化现象表明，运动可能是一种新的生理刺激，以抵消肥胖的肾上腺素调节的棕色募集脂肪细胞。(C)2012爱思唯尔有限公司版权所有。
Background and aim: Brown adipose tissue (BAT) plays a major role in body energy expenditure counteracting obesity and obesity-associated morbidities. BAT activity is sustained by the sympathetic nervous system (SNS). Since a massive activation of the SNS was described during physical activity, we investigated the effect of endurance running training on BAT of young rats to clarify the role of exercise training on the activity and recruitment state of brown cells.Methods and results: Male, 10-week-old Sprague Dawley rats were trained on a motor treadmill (approximately 60% of VO(2)max), 5 days/week, both for 1 and 6 weeks. The effect of endurance training was valuated using morphological and molecular approaches.Running training affected on the morphology, sympathetic tone and vascularization of BAT, independently of the duration of the stimulus.Functionally, the weak increase in the thermogenesis (no difference in UCP-1), the increased expression of PGC-1 alpha and the membrane localization of MCT-1 suggest a new function of BAT. Visceral fat increased the expression of the FOXC2, 48 h after last training session and some clusters of UCP-1 paucilocular and multilocular adipocytes appeared.Conclusion: Exercise seemed a weakly effective stimulus for BAT thermogenesis, but surprisingly, without the supposed metabolically hypoactive effects. The observed browning of the visceral fat, by a supposed white-to-brown transdifferentiation phenomena suggested that exercise could be a new physiological stimulus to counteract obesity by an adrenergic-regulated brown recruitment of adipocytes. (C) 2012 Elsevier B.V. All rights reserved.