IMMUNOCYTOCHEMICAL AND ULTRASTRUCTURAL STUDIES OF NEURONAL AND OLIGODENDROGLIAL CYTOPLASMIC INCLUSIONS IN MULTIPLE SYSTEM ATROPHY .1. NEURONAL CYTOPLASMIC INCLUSIONS
IMMUNOCYTOCHEMICAL AND ULTRASTRUCTURAL STUDIES OF NEURONAL AND OLIGODENDROGLIAL CYTOPLASMIC INCLUSIONS IN MULTIPLE SYSTEM ATROPHY .1. NEURONAL CYTOPLASMIC INCLUSIONS
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DOI：
10.1007/bf00310020
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发表时间：
1992-04-01
影响因子：
12.7
通讯作者：
INOSE, T
中科院分区：
文献类型：
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作者：
ARIMA, K;MURAYAMA, S;INOSE, T
对5例多系统萎缩（MSA）的神经元进行了组织学、免疫细胞化学和超微结构观察。所有病例均观察到嗜中性神经元胞质包涵体（NCIs）。分布于脑桥核、纹状体、下托、杏仁核、海马、齿状筋膜、黑质和下橄榄核。抗泛素抗体在退化的灰质和NCI中观察到许多增厚的神经突。一些NCI也被抗磷酸化神经丝抗体识别。从超微结构上看，NCI由直径为18 - 28 nm的颗粒相关细丝的网状结构组成，其与神经细丝混合。有髓纤维的轴浆中也有颗粒相关纤维。我们的研究表明NCI广泛分布于MSA的中枢神经系统。在轴突中形成颗粒相关细丝的相同病理过程也可能负责形成泛素阳性增厚的神经突。这些轴突改变，以及神经元核周的变化，可能在MSA的神经功能受损中发挥重要作用。
Neuronal alterations in five cases of multiple system atrophy (MSA) were investigated histologically, immunocytochemically and ultrastructurally. Argentophilic neuronal cytoplasmic inclusions (NCIs) were observed in all cases. They were distributed, in order of decreasing frequency, in the pontine nucleus, striatum, subiculum, amygdala, hippocampus, dentate fascia, substantia nigra and inferior olivary nucleus. Anti-ubiquitin antibodies visualized many thickened neurites in the degenerating gray matter as well as NCIs. Some NCIs were also recognized by anti-phosphorylated neurofilament antibodies. Ultrastructurally., NCIs consisted of a meshwork of granule-associated filaments, the diameter ranging from 18 to 28 nm, that were mixed with neurofilaments. The granule-associated filaments were also present in the axoplasm of myelinated fibers. Our studies demonstrate widespread distribution of NCIs in the central nervous system of MSA. The same pathological process that forms the granule-associated filaments in axons may also be responsible for the formation of ubiquitin-positive thickened neurites. These axonal alterations, as well as neuronal perikaryal changes, may play an important role in the impaired neuronal function in MSA.