Enhanced type X collagen expression in the extruded nucleus pulposus of the chondrodystrophoid dog
Enhanced type X collagen expression in the extruded nucleus pulposus of the chondrodystrophoid dog
复制标题
DOI：
10.1292/jvms.70.37
复制
发表时间：
2008-01-01
影响因子：
1.2
通讯作者：
Tagawa, Masahiro
中科院分区：
文献类型：
--
作者：
Itoho, Hisanori;Asou, Yoshinori;Tagawa, Masahiro
软骨营养不良犬早期髓核变性和钙化的原因尚不清楚，这一过程的潜在分子机制尚未明确。X型胶原是软骨内骨生长发育，尤其是基质钙化的关键分子之一。X型胶原与软骨营养不良犬关节盘退变和钙化的关系尚未得到研究。采用免疫组织化学方法，分析了X型胶原在软骨营养不良犬椎间盘退变和钙化过程中的表达。对照组5只犬(雌性4只，雄性1只，平均年龄1.3岁，比格犬)。对日本兽医动物科学大学收治的11例腰椎间盘突出症犬(雌犬1例，雄犬10例，平均年龄5.1岁，腊肠犬种)进行腰椎间盘摘除手术。所有突出的髓核标本均表现为细胞肥大和聚集，这是退变髓核的典型特征。退变髓核中X型胶原的相对表达(84.3+/-11.0%)明显高于对照髓核(5.4+/-5.4%)。我们的发现提示，X型胶原可能参与了软骨营养不良犬髓核的退变或钙化。
The causes of early degeneration and calcification of the nucleus pulposus in the chondrodystrophoid dog are poorly understood, and the underlying molecular mechanism of this process has not yet been clearly defined. Type X collagen is one of the key molecules in endochondral bone growth and development, especially matrix calcification. The relationship between type X collagen and disc degeneration and calcification in chondrodystrophoid dogs has not yet been studied. We analyzed the expression of type X collagen in degeneration and calcification of the intervertebral disc in chondrodystrophoid dogs, using type X collagen immunohistochemistry. Control intervertebral discs were collected from five dogs (4 female, 1 male, average age 1.3 years, beagle breed). Degenerated intervertebral discs were urgically removed from 11 canine patients with intervertebral disc extrusion (1 female, 10 male, average age 5.1 years, dachshund breed) in Nippon Veterinary and Animal Science University. All extruded disc samples showed hypertrophic changes and clustering of cells, typical features observed in the degenerated nucleus pulposus. The relative expression of type X collagen in the degenerated nucleus pulposus (84.3 +/- 11.0%) was significantly increased compared to the control nucleus pulposus (5.4 +/- 5.4%). Our findings suggest that type X collagen might contribute to the development of degeneration or calcification in the nucleus pulposus of the chondrodystrophoid dog.