Cloning and Functional Analysis of Pax6 from the Hydrothermal Vent Tubeworm Ridgeia piscesae.
Cloning and Functional Analysis of Pax6 from the Hydrothermal Vent Tubeworm Ridgeia piscesae.
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热液喷管虫 Ridgeia piscesae Pax6 的克隆及功能分析
DOI：
10.1371/journal.pone.0168579
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Yuan H;Wang W;Hu B;Pan C;Chen M;Ke L;Yang L;Chen J
Pax 6基因编码一种在多种动物谱系中对眼睛发育至关重要的转录因子。本文报道了深海热液盲管虫Ridgeia piscesae（RpPax 6）Pax 6基因的克隆和序列分析。推导的RpPax 6蛋白与其他物种的Pax 6蛋白具有广泛的序列同源性，并且包含配对结构域和完整的同源结构域。系统发育分析表明，该序列与环节动物门近缘种杜氏扁虫Platynereis dumerilii（P. dumerilii）的相应序列聚类。荧光素酶报告基因分析表明RpPax 6蛋白抑制了果蝇sine oculis（so）的转录。黑腹菌，干扰RpPax 6的C-末端。利用果蝇模型，我们发现RpPax 6的表达不能挽救ey 2突变体的小眼表型，只会导致更严重的无头表型。此外，RpPax 6表达诱导细胞凋亡，抑制细胞凋亡可以部分挽救RpPax 6诱导的无头表型。我们提供的证据表明RpPax 6至少起着两个作用：它阻断了眼发育级联反应中迟发性转录因子的表达，并促进了细胞凋亡。我们的研究结果表明，Pax 6功能的改变可能是导致vestimentiferan管虫眼缺失的可能原因之一。
The paired box 6 (Pax6) gene encodes a transcription factor essential for eye development in a wide range of animal lineages. Here we describe the cloning and characterization of Pax6 gene from the blind hydrothermal vent tubeworm Ridgeia piscesae (RpPax6). The deduced RpPax6 protein shares extensive sequence identity with Pax6 proteins from other species and contains both the paired domain and a complete homeodomain. Phylogenetic analysis indicates that it clusters with the corresponding sequence from the closely related species Platynereis dumerilii (P. dumerilii) of Annelida. Luciferase reporter assay indicate that RpPax6 protein suppresses the transcription of sine oculis (so) in D. melanogaster, interfering with the C-terminal of RpPax6. Taking advantage of Drosophila model, we show that RpPax6 expression is not able to rescue small eye phenotype of ey2 mutant, only to cause a more severe headless phenotype. In addition, RpPax6 expression induced apoptosis and inhibition of apoptosis can partially rescue RpPax6-induced headless phenotype. We provide evidence RpPax6 plays at least two roles: it blocks the expression of later-acting transcription factors in the eye development cascade, and it promotes cell apoptosis. Our results indicate alternation of the Pax6 function may be one of the possible causes that lead the eye absence in vestimentiferan tubeworms.
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影响因子：
64.8
作者：
HILL, RE;FAVOR, J;VANHEYNINGEN, V
通讯作者：
VANHEYNINGEN, V
影响因子：
5.3
作者：
Ikeda, K;Watanabe, Y;Kawakami, K
通讯作者：
Kawakami, K
影响因子：
10.5
作者：
Heanue, TA;Reshef, R;Tabin, CJ
通讯作者：
Tabin, CJ
DOI：
10.1007/bf00306706
发表时间：
1973-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
BRANDENBURGER, JL;WOOLLACOTT, RM;EAKIN, RM
通讯作者：
EAKIN, RM
影响因子：
11.4
作者：
Gehring, WJ;Ikeo, K
通讯作者：
Ikeo, K