Endotoxaemia and cytokine production in experimental colitis
Endotoxaemia and cytokine production in experimental colitis
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实验性结肠炎中的内毒素血症和细胞因子的产生
DOI：
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发表时间：
1995
影响因子：
9.6
通讯作者：
B. Rowlands
中科院分区：
文献类型：
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作者：
P. Neilly;K. Gardiner;S. J. Kirk;G. Jennings;N. Anderson;M. Elia;B. Rowlands
系统性内毒素血症是炎症性肠病的一个公认的特征，但其致病作用仍不确定。本研究在半抗原诱导的实验性结肠炎模型中检测了血浆内毒素和细胞因子浓度以及急性期蛋白反应。在三硝基苯磺酸乙醇诱导结肠炎（TNBS‐E）后第2、8和14天，检测血浆内毒素、免疫球蛋白（Ig） G和IgM内毒素核心抗体（EndoCAb）、肿瘤坏死因子（TNF）、白细胞介素（IL） 6和α2‐巨球蛋白（α2M）浓度和结肠宏观炎症评分。与对照组相比，在所有时间点均有显著的结肠炎症（P< 0.0001）。经TNBS‐E处理的动物在所有时间点的内毒素浓度均升高（P< 0.04）。在TNBS‐E‐处理的动物中，EndoCAb浓度在第2天降低（P< 0.0001），随后升高。TNBS - E -处理动物的IL - 6和α2M浓度升高，但TNF浓度无显著变化。内毒素浓度与宏观炎症评分、IL - 6和α2M浓度相关。EndoCAb浓度与这些参数之间的相关性不太一致。这些结果表明，内毒素是这个实验性结肠炎模型的全身反应的中介。
Systemic endotoxaemia is a well recognized feature of inflammatory bowel disease but its pathogenic role remains uncertain. This study examined plasma endotoxin and cytokine concentrations and the acute‐phase protein response in a hapten‐induced model of experimental colitis. On days 2, 8 and 14 after induction of colitis with trinitrobenzenesulphonic acid in ethanol (TNBS‐E), plasma endotoxin, immunoglobulin (Ig) G and IgM endotoxin‐core antibody (EndoCAb), tumour necrosis factor (TNF), interleukin (IL) 6 and α2‐macroglobulin (α2M) concentrations and colon macroscopic inflammation score were determined. At all time points there was significant colonic inflammation when compared with control values (P<0·0001). Animals treated with TNBS‐E had raised concentrations of endotoxin at all time points (P<0·04). In TNBS‐E‐treated animals EndoCAb concentrations were reduced on day 2 (P<0·0001) and later increased. There were increases in IL‐6 and α2M concentrations in TNBS‐E‐treated animals but no significant change in TNF concentrations. Endotoxin concentrations correlated with macroscopic inflammation score, IL‐6 and α2M concentrations. There was a less consistent correlation between EndoCAb concentrations and these parameters. These results suggest that endotoxin is a mediator of the systemic response in this model of experimental colitis.
影响因子：
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作者：
Mitchell P. Fink;Jan B. Antonsson;Hailong Wang;H. R. Rothschild
通讯作者：
Mitchell P. Fink;Jan B. Antonsson;Hailong Wang;H. R. Rothschild